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of the North Caucasus
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Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137
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Influence of blockers of slow calcium channels on the processes of proliferation and collagenogenesis of fibroblasts in the formation of spices in the abdominal cavity

[Original research] [Experimental medicine]
Lyudmila Kuzyakova; Eleanor Stepanova; Sergey Skalsky; Marina Alekseevna Ogay; Tamara Nikolaevna Glizhova; Alexander Vladimirovich Lokarev; Tatiana Fedorovna Sokolova;

Based on the results of the studies, it was found that the modeling of hemoperitoneum at the adhesion stage is accompanied by an increase in the proliferation of fibroblasts: an increase in the mitotic index by a factor of 5, the level of the Ki-67 proliferation marker by a factor of 8, and the enhancement of their collagen-synthetic function: a 4-fold increase in the formation of protein-bound oxyproline. Verapamil and diltiazem reduce the excessive activity of fibroblasts at the stage of formation of adhesions by regulating their proliferative and collagen-synthetic functions, reducing the number of mitoses by 58.5 %, the number of Ki-67+ cells by 59.2 %, the excess production of oxyproline by 66.7 %. The normalizing effect of verapamil on fibroblasts is 1.8 times higher than that of diltiazem. Nifedipine has no antiproliferative effect on cells.

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References:
1. Zhang Y., Zhao X., Chang Y., Zhang Y., Chu X. [et al.]. Calcium channel blockers ameliorate iron overloadassociated hepatic fibrosis by altering iron transport and stellate cell apoptosis. Toxicology and Applied Pharmacology. 2016;301:50-60. https://doi.org/10.1016/j.taap.2016.04.008
2. Alekseev A. A., Sulima A. N. Sovremennyye predstavleniya ob etiologii i patogeneze spayechnogo protsessa v malom tazu u zhenshchin reproduktivnogo vozrasta. Medicinskii Westnik Yuga Rossii. – Medical Bulletin of the South Russia. 2016;(1):4-14. (In Russ.)
3. Alkhamesi N. A., Schlachta C. M. The role of aerosolized intraperitoneal heparin and hyaluronic acid in the prevention of postoperative abdominal adhesions. Surgical Endoscopy. 2013;27(12):4663-4669. https://doi.org/10.1007/s00464-013-3102-5
4. Fortin C. N., Saed G. M. Diamond MP. Predisposing factors to post-operative adhesion development. Human Reproduction Update. 2015;21(4):536-551. https://doi.org/10.1093/humupd/dmv021
5. Roman H., Bourdel N., Canis M., Rigaud J., Delavierre D. [et al.]. Adhesions and chronic pelvic pain. Progrès en Urologie. 2010;20(12):1003-9. https://doi.org/10.1016/j.purol.2010.08.003

Keywords: chronic fibroproliferative diseases, postoperative adhesions, scars, fibroblasts, extracellular matrix


Founders:
Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy