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[Original research] [Infectious diseases]
Olga Viktorovna Bobrysheva; Sergey Vladimirovich Pisarenko; Dmitriy Kovalev; Evgeniy Eremenko; Alla Ryazanova; Olga Viktorovna Semenova; Diana Vasilyevna Ulshina; Alexandr Kulichenko;
The objective was to present the data of the molecular genetic analysis of Bacillus anthracis strains isolated during outbreaks of anthrax in the Republic of Dagestan. For phylogenetic analysis, genomic sequences of 6 B. anthracis strains and 266 B. anthracis genomic sequences from the international GenBank database were used. As a result of genetic analysis, it was found that the strains isolated in 1957 and 1963 belong to the main genetic line B, branch B.Br.002 and have a high degree of genetic relationship with strains from Western Siberia, which indicates their common origin. Isolates distinguished in 2019 belong to the TEA Br.008/011 group, A.Br.118 branch. These strains form a separate branch and are closely related to the «STI» clade. The data obtained can be used in the differentiation of strains during the investigation of outbreaks of anthrax.
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Keywords: Bacillus anthracis, genome-wide sequencing, genome-wide analysis of single nucleotide polymorphism, comparative genomics