logo
Medical news
of the North Caucasus
Scientific journal
Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137
rus
русский
eng
english

Site search



Correspondence address
310 Mira Street, Stavropol, Russia, 355017

Tel
+7 8652 352524; +7 8652 353229.

Fax
+7 8652 352524.

E-mail
medvestnik@stgmu.ru

Comparison of the effectiveness of various sulphur-containing hepatoprotectors against chronic alcoholization

[Experimental medicine]
Ilya Bykov; Hristina Berberidi; Konstantin Popov; Galina Ermakova; Igor Tsymbalyuk; Elena Evgenevna Esaulenko; Yana Evgenievna Denisova; Erustam Adamovich Azimov;

Studies on the treatment of intoxication caused by prolonged use of alcohol are necessary. Herein, the influence of ademetionine, methionine and lipoic acid under various administration schemes on the course of chronic alcoholization was compared. The study was performed on 125 white nonlinear male rats (initial body mass: 220–250 g) divided into seven groups. Groups 2–7 underwent alcoholization for two months. The rats of groups 3–7 were administered ademetionine, methionine or lipoic acid. We determined herein that ademetionine injections had the most significant hepatoprotective and antioxidant effects, and they maintained the mitochondria in an adequate functional state. Methionine administration showed no cytoprotective effect, and it was characterized by mitochondrial dysfunction and a more significant increase in the activity of hepatic cytolysis markers. The lipoic acid injections had a significant antioxidant effect, but their cytoprotective effects were mild. On the basis of our findings, we concluded that the substances tested here mainly improved metabolic disorders induced by chronic alcoholization via maintenance of the detoxification and biosynthetic functions of the liver.

Download

References:
1. Bykov I. М., Popov К. А., Egorova I. А., Storozhuk A. P.Assessment of indicators of the thiol metabolism of blood plasma of patients with inflammatory diseases of the small pelvis organs at antioxidant correction. Medical news of North Caucasus. 2018;13(2):402-406. https://doi.org/10.14300/mnnc.2018.13062
2. Popov К. А., Bykov I. М., Tsymbalyuk I. Yu., Bykov М. I.,Sidorenko A. N. [et al.] Changes in state of the thiol linkages of an antioxidant system during ischemia and reperfusion,against a background of vascular exclusion in the rat liver. Medical news of North Caucasus. 2018;13(3):525-529. https://doi.org/10.14300/mnnc.2018.13096
3. Jung Y. S. Metabolism of sulfur-containing amino acids in the liver: A link between hepatic injury and recovery. Biol.Pharm. Bull. 2015;38(7):971-974. https://doi.org/10.1248/bpb.b15-00244
4. Dolgareva S. A., Sorokin A. V., Konoplya N. A., Bushmina O. N., Bystrova N. A., Ovod A. I. The use of immunomodulators, antioxidants and hepatoprotectors for the correction of the liver, erythrocites and the immune system disorders in chronic ethanol intoxication. Biomedical Chemistry. 2018;64(4):360-367. https://doi.org/10.18097/PBMC20186404360
5. Raikhelson K. L., Kondrashina E. A. Аdеmethionine in the treatment of fatigue in liver diseases: a systematic review. archive. 2019;91(2):134-142. https://doi.org/10.26442/00403660.2019.02.000130
6. Onaolapo O. J., Adekola M. A., Azeez T. O., Salami K.,Onaolapo A. Y. l-Methionine and silymarin: A comparison of prophylactic protective capabilities in acetaminopheninduced injuries of the liver, kidney and cerebral cortex. Biomed. Pharmacother. 2017;85:323-333. https://doi.org/10.1016/j.biopha.2016.11.033
7. Hosseinpour-Arjmand S., Amirkhizi F., Ebrahimi-Mameghani M. The effect of alpha-lipoic acid on inflammatory markers and body composition in obese patients with non-alcoholic fatty liver disease: A randomized, double-blind, placebocontrolled trial. J. Clin. Pharm. Ther. 2019;44(2):258-267. https://doi.org/10.1111/jcpt.12784
8. Valdecantos M. P., Pérez-Matute P., Prieto-Hontoria P., Moreno-Aliaga M. J., Martínez J. A. Impact of dietary lipoic acid supplementation on liver mitochondrial bioenergetics and oxidative status on normally fed Wistar rats. It. J. Food Sci. Nutr. 2019;15:1-11. https://doi.org/10.1080/09637486.2019.1572716
9. Belenichev I. F., Kucher T. V. The deprivation of glutathione system in the cytosol and mitochondria of rat brain with chronic alcohol intoxication: protective effects of thiol antioxidants. Bulletin of problems biology and medicine. 2016;1(4):111-116.
10. Shulkin L. M., Kosenko N. A., Kosenko V. G., Ageev M. I., Shulkin M. Z. Clinical overview remission of alcohol dependence. Kuban Scientific Medical Journal. 2015;2:153-159.
11. Volchegorskiĭ I. A., Rassokhina L. M., Miroshnichenko I. Yu. Insulin-potentiating action of antioxidants in experimental diabetes mellitus. Problemi Endocrinologii. – Problems of endocrinology. 2010;56 (2):27-35.
12. Karpishchenko A. I. Handbook. Medical Laboratory Technology. Sankt-Petersburg: Intermedika, 2002.
13. Egorova M. V., Afanasyev S. A. Isolation of mitochondria from cells and tissues of animals and human: Modern methodical approaches. Sibirskij medicinskij žurnal. - Siberian Medical Journal. 2011;26(1):22-28.
14. Cheshchevik V. T. Mitochondrial membrane potential of rat hepatocytes under toxic injury. Izvestiya Nacional’noj akademii nauk Belarusi. Seriya biologicheskih nauk. – Proceedings of the National Academy of Sciences of Belarus,Biological Series. 2010;2:75-80.
15. Zorova L. D., Popkov V. A., Plotnikov E. J., Silachev D. N., Pevzner I. B. [et al.] Roles of Mitochondrial Membrane Potential. Membrane and Cell Biology. 2017;34(6):93-100. https://doi.org/10.7868/S0233475517060020

Keywords: alcoholization, oxidative stress, antioxidant system, methionine, lipoic acid, ademetionine


Founders:
Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy