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https://doi.org/10.14300/mnnc.2018.13059

Evaluation of the free radicals production rate in tissue -engineered constructions using EPR -srectroscopy

[Experimental medicine]
Ilya Bykov; Elena Gubareva; Еlena Kuevda; Alexander Sotnichenko; Ivan Gumenyuk; Oksana Lyasota; Sergey Bolotin; Stepan Dzhimak;

Tissue engineering grows rapidly nowadays and may turn to a promising alternative to organs and tissues transplantation since it is devoid of the essential disadvantages of transplantology such as lack of donor material, complexity of its matching as well as its delivery and preservation, and life-long immunosuppressive therapy. Decellularization is one of the best known methods of biological scaffolds obtainment for the creation of tissue-engineered organs and tissues. In the present paper we considered biophysical criteria of decellularization on the nonhuman primates’ esophagus and diaphragm decellularization based on the evaluation of the free radicals production rate in native and decellularized tissues using EPR-spectroscopy in a complex quality assessment of obtained biological matrices as well as the possibility of their cryopreservation for the following
transportation.

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Keywords: regenerative medicine, tissue engineering, decellularization, EPR-spectroscopy, scaffold