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ISSN 2073-8137

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The study of Arg25Pro polymorphism gene of transforming growth factor β1 in children with bronchial asthma

Alexander Lebedenko; Tatiana Shkurat; Elena Mashkina; Olga Semernik; Tatiana Drejzina;

Objective: to study the role of polymorphism of Arg25Pro gene TGFβ1 in the pathogenesis of asthma in children. We studied the association of Arg25Pro polymorphism of the TGFβ gene with the risk of developing asthma in children. The results of the genetic analysis are compared with the content of TGFβ1 in the blood serum of patients. When studying the Arg25Pro polymorphism of the TGFβ1 gene, it was noted that among the examined patients homozygotes over the Arg25 allele predominate. The frequency of heterozygotes Arg25Pro among the group of children suffering from asthma is 3.08 times lower in comparison with healthy children. The association of polymorphism of Arg25Pro gene TGFβ1 with an increased risk of asthma in children has been established. It was shown that in patients with the ArgArg genotype of the TGFβ1 gene, the concentration of TGFβ1 in the blood serum was increased (132.08±77.28 pg/ml), compared with heterozygotes (88.47±86.26 pg/ml) or patients with ProPro genotype (94.77±92.48 pg/ml).
The association of the Arg25Pro polymorphism of the TGFβ1 gene with an increased risk of developing asthma in children has been established. The relationship of this gene polymorphism with increased synthesis of TGFβ1 is shown.


1. Ilchenko S. I., Fialkovskaya A. A., Ivanus S. G. The value of serum transforming growth factor level in bronchial remodeling in adolescent smokers. Evrazysky Soyuz Uchenykh (ESU). meditsinskiye nauki. – Eurasian Union of scientists (EU). Medical science. 2015;10(19):224- 230. (In Russ.).
2. Lebedenko A. A., Semernik O. E. Neurohumoral aspects of bronchial asthma exacerbation in children. Pulmonologiya. – Pulmonology. 2013;5:36-39. (In Russ.). https:// doi.org/10.18093/0869-0189-2013-0-5-36-39
3. Lebedenko A. A., Shkurat T. P., Semernik O. E., Mashkina E. V., Dreizina T. K. Polymorphism of the gene of the growth factor of the vascular endothelium in children of the Rostov region, suffering from bronchial asthma. Valeologiya. – Valeology. 2016;4:20-25. (In Russ.). https://doi.org/10.18522/2218-2268-2016- 4-20-25
4. National program «Bronchial asthma in children. The strategy of treatment and prevention». 5th ed., rev. and additional. Moscow: Izd. house «Atmosphere», 2017. (In Russ.).
5. Razvodovskaya A. V., Cherkashina I. I., Nikulina S. Yu., Shestovitsky V. A., Voevoda M. I., Maksimov V. N. Study of the association of single nucleotide polymorphism rs1800470 of the gene transforming growth factor beta 1 (TGF-β1) with the risk of developing bronchial asthma. Sibirskoye meditsinskoye obozreniye. – Siberian medical review. 2014;2:17-22. (In Russ.).
6. Semernik O. E., Lebedenko A. A. Features of vegetative response in children with bronchial asthma in the period of exacerbation of the disease. Vestnik Rossiyskoy akademii meditsinskikh nauk. – Bulletin of the Russian Academy of Medical Sciences. 2015;70(2):222-226. (In Russ.). https://dx.doi.org/10.15690/vramn.v70i2.1316
7. Lee S. Y., Lee Y. H., Shin C., Shim J. J., Kyung H. K., Se H. Y., Kwang H. I. Association of asthma severity and bronchial hyperresponsiveness with a polymorphism in the cytotoxic T-lymphocyte antigen-4 gene. Chest. 2002;122(1):171-176.
8. Li H., Wu H., Sienra-Monge J. J., Ramírez-Aguilar M., del Río-Navarro B. E., del Lara-Sánchez I. C., Kistner E. O., Gjessing H. K., London S. J. Genetic polymorphisms in transforming growth factor beta-1 (TGFB1) and childhood asthma and atopy. Hum. Genet. 2007;121(5):529-538. https://doi.org/10.1007/s00439-007-0337-z
9. Salam M. T., Gauderman W. J., McConnell R., Lin P. C., Gilliland F. D. Transforming growth factor- 1 C-509T polymorphism, oxidant stress, and early-onset childhood asthma. Am. J. Respir. Crit. Care Med. 2007;176(12):1192-1199. https://doi.org/10.1164/rccm.200704-561OC
10. Sharma S., Raby B. A., Hunninghake G. M., Soto-Quirós M., Avila L., Murphy A. J., Lasky-Su J., Klanderman B. J., Sylvia J. S., Weiss S. T., Celedón J. C. Variants in TGFB1, dust mite exposure, and disease severity in children with asthma. Am. J. Respir. Crit. Care Med. 2009;179:356- 362. https://doi.org/10.1164/rccm.200808-1268OC
11. Shkurat T. P., Lebedenko A. A., Mashkina E. V., Semernik O. E., Dreyzina T. K. Vascular Endothelial Growth Factor: Genetic Aspects in Children with Asthma in the Rostov Region. The Online Journal of Health and Allied Sciences. 2016;15(4). Available at: www.ojhas.org/issue60/ 2016-4-7.html.

Keywords: bronchial asthma, children, gene, polymorphism, transforming growth factor β1 (TGFβ1)

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy