• Main page
• About the journal
• Editorial board
• Subscription
• Information for contributors
• Editorial and Peer Review
• Issues archive
• Authors
• Articles
  • By alphabet
  • By category
• Blogs and comments

The journal is included into The list of leading scientific periodicals.

The journal is included into VINITI database and is registered in Electronic scientific library.

The journal is indexed by SCOPUS, Ulrich's International Periodicals Directory.

EBSCO

https://doi.org/10.14300/mnnc.2015.10040

Gelatinase B and magnesium in the development of experimental gastric ulcer

[Experimental medicine]
Lyudmila Rogova; Natalya Shesternina;

Purpose of study was to determine the specific number and expression activity of gelatinase B-positive cells in the ulcerous defect, and the magnesium levels in the concentrated red cells obtained from various locations in rats with experimental acetate gastric ulcer. The experiment was performed on 18 white Wistar rats. The study of the specific number and gelatinase B expression activity was done through immunohistochemistry (Novocastra, NCL–ММР9, for the paraffin blocks, working dilution 1:40); the content of magnesium in red blood cells through the reaction with titan yellow. The study allows concluding that in the site of experimental ulcerous defect there is an increase in the number and the expression activity оf gelatinase B-positive cells in the surface epithelium, with even a higher degree of increase in the gastric LPS (cellular component) against lowered magnesium levels in the concentrated red cells.

Download

References:
1. Arunin L.I., Kapuller L.L., Isakov V.A. Morphological diagnosis of diseases of the stomach and intestines. Triada-Х, 1998:496p.
2. Bacon C., Ming-Qing Du, Dogan A. Mucosa-associated lymphoid tissue (MALT) lymphoma: a practical guide for pathologists. J. Clin. Pathol. 2007;60:361–372.
3. Ivashkin V.Т. Meditsinskii Vestnik Severnogo Kavkaza. – Medical News of North Caucasus. 2006;19(362):9–10.
4. Kamishnikov V.S. Handbook of clinical and biochemical research laboratory diagnostics. Мoscow: МЕDpressinform. 2004.
5. Korzhevsky D.E. Basics of histologic techniques. Moscow: Meditsina, 2010:234p.
6. Okabe S., Amagase K. An overview of acetic acid ulcer models. The history and state of the art of peptic ulcer research. Biol. Pharm. Bull. 2005;8(28):1321-1341.
7. Okorokov А.N. Diagnosis of diseases of the internal organs: Volume 1. Diagnosis of diseases of the digestive system. Мoscow: Med.lit. 2002:560p.
8. Parks W.C., Wilson C.L., López-Boado Y.S. Matrix metalloproteinases as modulators of inflammation and innate immunity. Nature Reviews Immunology. 2004;8(4):617–629.
9. Povetkina V.N., Rogova L.N. Vestnik novich meditsinskich technologiy. – Bulletin of new medical technologies. 2011;2(18):83-86.
10. Rubin H. The logic of the Membrane, Magnesium, Mitosis (MMM) model for the regulation of animal cell proliferation. Science. 2003;261:1280-1281.
11. Staravoitov V.А., Rogova L.N., Stacenko М.Е. Vestnik Rossiiskogo universiteta druzhbi narodov (Medicina) – Bulletin of the Russian Peoples’ Friendship University (medicine series). 2008;7:532-534.
12. Troshin I.Yu., Gromova О.А. Kardiologiya – Cardiology. 2008;10:14-21.

Keywords: experimental acetate gastric ulcer, gelatinase B, magnesium