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https://doi.org/10.14300/mnnc.2026.00010

In vivo study of the embryocytotoxicity of implant materials based on titanium and zirconium alloys using the chick embryo chorioallantoic membrane model

[Experimental medicine]
Alexander Dolgalev; Maria Sergeevna Bagdasaryan; Nikolay Nikolaevich Koval; Yuri Fedorovich Ivanov; Maxim Sergeevich Vorobyov; Alesya Khripunova; David Zurabovich Choniashvili; Kristina Parkhomenko;

The biocompatibility of samples for implantation surgery based on various titanium and zirconium alloys was studied. The study model was the chorioallantoic membrane (CAM) of the chicken embryo, as one of the most sensitive methods for studying the primary biocompatibility reaction of materials. On the 9th and 13th days of embryogenesis, morphological and vascular changes in CAM were recorded. A high biocompatibility of titanium dioxide (TiO2) and titanium-aluminum-vanadium alloy (BT6) samples was revealed, which showed the preservation of a dense branched vascular network and the absence of signs of vascular degradation. The titanium-niobium-zirconium-tantalum alloy (TiNbZrTa) demonstrated a pronounced effect of vascular network suppression. The study revealed the most promising coatings for application to the surface of implants, for which it is necessary to continue further experiments in vivo.

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Keywords: titanium alloys, biocompatibility, angiogenesis, chorioallantoic membrane, implantation materials