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https://doi.org/10.14300/mnnc.2026.00009

Effect of different NO-therapies on rat plasma metabolic response in experimental thermal injury

[Experimental medicine]
Lida Kovaleva; Andrey Martusevich; Anna Soloveva; Levon Dilenyan; Vladimir Kononets;

The objective of the study is a comparative assessment of the state of oxidative metabolism and crystallogenic properties of rat blood plasma with contact thermal burns under experimental therapy with various sources of nitric oxide (II). A preclinical experimental randomized study was conducted on 50 sexually mature male Wistar rats weighing between 200 and 250 grams. The animals were divided into 5 groups of 10 individuals each using the «envelope» method. Group 1 was intact (without any manipulation), while groups 2, 3, 4, and 5 had a simulated thermal injury in the form of a contact thermal burn. To study the balance of pro- and antioxidant systems in blood plasma, the intensity of lipid peroxidation and the total activity of antioxidant systems were investigated, the level of malondialdehyde was determined, and the crystallogenic properties of the biological fluid were assessed. In erythrocytes, the activity of superoxide dismutase and catalase was evaluated. It was demonstrated that the application of nitric oxide, regardless of its administration form, creates conditions for accelerated and more complete resolution of oxidative stress phenomena and inhibition of blood crystallogenic activity induced by the formation of burn disease in an experimental model. At the same time, each of the studied NO therapy regimens has its own characteristic features: the inhalation variant has the most optimal effect on the proteome of the biological environment, the application of a physiological donor of the compound maximally normalizes organo-mineral homeostasis,
and their combination allows for the most significant stimulation of the antioxidant systems of animal blood plasma.

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Keywords: nitrogen monoxide, thermal injury, burn disease, pathogenesis, oxidative stress, biocrystallomics