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Prooxidant preconditioning of ischemic -reperfusion liver damage in experiment

[Experimental medicine]
Konstantin Popov; Ilya Bykov; Igor Tsymbalyuk; Rustam Adamovich Azimov; Mikhail Bykov; Yana Evgenievna Denisova; Angela Nikolaevna Stolyarova; Elena Evgenevna Esaulenko;

In the development of the protective effects of ischemic preconditioning, various signaling pathways, including those mediated by reactive oxygen species, play an outstanding role. The purpose of the study was to evaluate the effect of preliminary administration of tert-butyl hydroperoxide on the development of liver damage in rats during ischemiareperfusion modeling. The study was carried out on 55 white non-linear male rats, divided into 3 groups: the control group (1st group), not exposed to any influences; Groups 2 and 3, where rats underwent modeling of vascular exclusion of the liver for 20 minutes followed by 3 hours of reperfusion. The animals of the experimental groups were injected intraperitoneally with 1 ml of saline (Group 2) or 1 ml of 0.5 % solution of tert-butyl hydroperoxide (group 3) intraperitoneally. As a result of the studies, it was revealed that against the background of preliminary administration of tert-butyl hydroperoxide, the activity of ALT, AST, LDH and glutathione-S-transferase was 1.5–4.5 times lower than the values of similar indicators in animals of the 2nd group. Thus, the scientific hypothesis about the possibility of preconditioning of ischemia-reperfusion liver damage using prooxidant factors was confirmed.

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Keywords: ischemia, reperfusion, ischemia-reperfusion syndrome, prooxidant factors, oxidative stress, preconditioning


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