logo
Medical news
of the North Caucasus
Scientific journal
Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137
rus
русский
eng
english

Site search



Correspondence address
310 Mira Street, Stavropol, Russia, 355017

Tel
+7 8652 352524; +7 8652 353229.

Fax
+7 8652 352524.

E-mail
medvestnik@stgmu.ru

Influence of urokinase gene knockout in C57BL/6-Plautmi.IBug-This Plau6FDhu/GFDhu mice on levels of brain neurotrophin receptors in experimental melanoma developing in presence of chronic neurogenic pain

[Experimental medicine]
Elena Frantsiyants; Valeriya Bandovkina; Irina Kaplieva; Irina Valerievna Neskubina; Ekaterina Igorevna Surikova; Natalia Cheryarina; Lydia Trepitaki; Natalya Sergeevna Lesovaya;

The results of assessing the dynamics of the content of estrogen receptors (ER-α and β), androgens (RA) and progesterone (RP4) in the brain of mice of the C57BL/6 and C57BL/6-PlautmI.IBug-This Plau6FDhu/GFDhu lines knocked out by the urokinase gene, with the growth of experimental melanoma B16/F10 in an independent variant and associated with chronic neurogenic pain (CNP) in 80 mice are presented. The level of neurosteroid receptors in the brain is probably related to tumor volume. A sharp increase in the volume of tumors in urokinase knockout mice under the influence of CNP was accompanied by a decrease in the ER-α+ER-β/RA ratio in the brain as compared with the indices in knockout mice with spontaneous melanoma growth. In C57Bl/6 females, CNP had no significant effect on tumor volumes and the ER-α+ER-β/RA ratio.

Download

References:
1. Grube M., Hagen P., Jedlitschky G. Neurosteroid Transport in the Brain: Role of ABC and SLC Transporters. Frontiers in Pharmacology. 2018;9:354. https://doi.org/10.3389/fphar.2018.00354
2. Ratner M. H., Kumaresan V., Farb D. H. Neurosteroid Actions in Memory and Neurologic/Neuropsy chiatric Disorders. Frontiers in Endocrinology. 2019;10:169. https://doi.org/10.3389/fendo.2019.00169
3. Mendell A. L., MacLusky N. J. Neurosteroid Metabolites of Gonadal Steroid Hormones in Neuroprotection: Implications for Sex Differences in Neurodegenerative Disease. Front Molecular Neuroscience. 2018;11:359. https://doi.org/10.3389/fnmol.2018.00359
4. McEwen B. S., Milner T. A. Understanding the broad influence of sex hormones and sex differences in the brain. Journal of Neuroscience Research. 2017;95:24-39. https://doi.org/10.1002/jnr.23809
5. Meyer L., Taleb O., Patte-Mensah C., Mensah-Nyagan A. G. Neurosteroids and neuropathic pain management: Basic evidence and therapeutic perspectives. Frontiers in Neuroendocrinology. 2019;55:100795. https://doi.org/10.1016/j.yfrne.2019.100795
6. McEwen B. S. Hormones and behavior and the integration of brain-body science. Hormones and Behavior. 2020;119:104619. https://doi.org/10.1016/j.yhbeh.2019.104619
7. Yepes M. Urokinase-type plasminogen activator is a modulator of synaptic plasticity in the central nervous system: implications for neurorepair in the ischemic brain. Neural Regeneration Research. 2020;15(4):620-624. https://doi.org/10.4103/1673-5374.266904
8. Frantsiyants E. M., Bandovkina V. A., Kaplieva I. V., Cheryarina N. D., Surikova E. I. [et al.]. Influence of malignant growth and chronic neurogenic pain on neurosteroid levels in rat brain. Biomeditsinskaya khimiya. – Biomedical Chemistry. 2020;66(2):2-11. (In Russ.).
9. Henry N. L., Hayes D. F., Ramsey S. D., Hortobagyi G. N., Barlow W. E., Gralow J. R. Promoting quality and evidence-based care in early-stage breast cancer follow-up. Journal of the National Cancer Institute. 2014;106(4):dju034. https://doi.org/10.1093/jnci/dju034
10. Del Río J. P., Alliende M. I., Molina N., Serrano F. G., Molina S., Vigil P. Steroid Hormones and Their Action in Women’s Brains: The Importance of Hormonal Balance. Frontiers in Public Health. 2018;6:141. https://doi.org/10.3389/fpubh.2018.00141
11. Azcoitia I., Barreto G. E., Garcia-Segura L. M. Molecular mechanisms and cellular events involved in the neuroprotective actions of estradiol. Analysis of sex differences. Frontiers in Neuroendocrinology. 2019;55:100787. https://doi.org/10.1016/j.yfrne.2019.100787
12. Almey A., Milner T. A., Brake W. G. Estrogen receptors in the central nervous system and their implication for dopamine-dependent cognition in females. Hormones and Behavior. 2015;74:125-138. https://doi.org/10.1016/j.yhbeh.2015.06.010
13. Marrocco J., McEwen B. S. Sex in the brain: hormones and sex differences. Dialogues in Clinical Neuroscience. 2016;18(4):373-383.
14. Frantsiyants E. M., Kaplieva I. V., Surikova E. I., Neskubina I. V., Bandovkina V. A. [et al.]. Effect of urokinase gene-knockout on growth of melanoma in experiment. Sibirskiy Nauchny Meditsinskiy Zhurnal. – Siberian Scientific Medical Journal. 2019;39(4):62-70. (In Russ.). https://doi.org/10.15372/SSMJ20190408

Keywords: urokinase gene-knockout mice, chronic neurogenic pain, В16/F10 melanoma, neurosteroid receptors


Founders:
Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy