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Influence of D-asparagine on the proliferative activity of human dermal fibroblasts

[Experimental medicine]
Yulia Anatolievna Sergeeva; Artem Ivanovich Trofimenko; Tatiana Vladimirovna Fedorenko; Irina Gilevich; Elena Anatolievna Kolomiytseva; Azamat Kade;

The effect of D-asparagine at a concentration of 0.013–0.13 g/l on proliferative and metabolic activity, as well as the morphology of human dermal fibroblasts, was studied. It was shown that D-asparagine, when exposed for 2–6 hours at a concentration of 0.013–0.13 g/l, rendered a stimulating effect on the proliferative activity of human fibroblasts, and with a 24–48 hour exposure, the opposite influence was marked. Moreover, the antiproliferative effect of D-asparagine at a concentration of 0.13 g/l was associated with a substantial changes in cell morphology. Applying of tetrazolium methods for evaluation of changes in the metabolic activity of cells in the case of D-asparagine does not seem indicative. Thereby, the inverse-proportional relationship between the exposure time of D-asparagine (2–48 hours) and the proliferative activity of human dermal fibroblasts was shown.


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Keywords: D-asparagine, dermal fibroblast, proliferative activity, human

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy