Medical news
of the North Caucasus
Scientific journal
Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137

Site search

Correspondence address
310 Mira Street, Stavropol, Russia, 355017

+7 865 2352511, +7 865 2353229.


Influence of D-asparagine on the proliferative activity of human dermal fibroblasts

[Experimental medicine]
Yulia Anatolievna Sergeeva; Artem Ivanovich Trofimenko; Tatiana Vladimirovna Fedorenko; Irina Gilevich; Elena Anatolievna Kolomiytseva; Azamat Kade;

The effect of D-asparagine at a concentration of 0.013–0.13 g/l on proliferative and metabolic activity, as well as the morphology of human dermal fibroblasts, was studied. It was shown that D-asparagine, when exposed for 2–6 hours at a concentration of 0.013–0.13 g/l, rendered a stimulating effect on the proliferative activity of human fibroblasts, and with a 24–48 hour exposure, the opposite influence was marked. Moreover, the antiproliferative effect of D-asparagine at a concentration of 0.13 g/l was associated with a substantial changes in cell morphology. Applying of tetrazolium methods for evaluation of changes in the metabolic activity of cells in the case of D-asparagine does not seem indicative. Thereby, the inverse-proportional relationship between the exposure time of D-asparagine (2–48 hours) and the proliferative activity of human dermal fibroblasts was shown.


1. Chitanava T., Egiev I., Chechelian V., Dzhopua M., Trofimenko A. [et al.]. The Influence of D-Asparagine on the Glial Cicatrix Formation in Experimental Spinal Stroke. American Journal of Clinical and Experimental Medicine. 2017;5(3):93-96.https://doi.org/10.11648/j.ajcem.20170503.15
2. Sergeeva Yu. A., Kade A. Kh., Gaivoronskaya T. V., Trofimenko A. I., Basov A. A. [et al.]. Improvement of lipofilling efficacy in treatment of experimental dermal scars by using D-asparagine. Krymsky zhurnal eksperimentalnoy i klinicheskoy meditsiny. – Crimea Journal of Experimental and Clinical Medicine. 2019;9(3):41-47. (In Russ.).
3. D’aniello A. D-Aspartic acid: an endogenous amino acid with an important neuroendocrine role. Brain research reviews. 2007;53(2):215-234. https://doi.org/10.3390/nu11092205
4. de Rosa V., Secondo A., Pannaccione A., Ciccone R., Formisano L. [et al.]. D-Aspartate treatment attenuates myelin damage and stimulates myelin repair. EMBO Molecular Medicine. 2019;11(1):e9278. https://doi.org/10.15252/emmm.201809278
5. Genchi G. An overview on D-amino acids. Amino Acids. 2017;49(9):1521-1533.
6. Du S., Wang Y., Alatrash N., Weatherly C.A., Roy D. [et al.]. Altered profiles and metabolism of l-and d-amino acids in cultured human breast cancer cells vs. non-tumorigenic human breast epithelial cells. Journal of pharmaceutical and biomedical analysis. 2019;164:421-429. https://doi.org/10.1016/j.jpba.2018.10.047
7. Sacchi S., Cappelletti P., Murtas G. Biochemical Properties of Human d-amino Acid Oxidase Variants and Their Potential Significance in Pathologies. Frontiers in Molecular Biosciences. 2018;5:55. https://doi.org/10.3389/fmolb.2018.00055
8. D’Aniello A., D’Onofrio G., Pischetola M., D’Aniello G., Vetere A. [et al.]. Biological role of D-amino acid oxidase and D-aspartate oxidase. Effects of D-amino acids. Journal of Biological Chemistry. 1993;268(36):26941-26949.
9. Fujino T., Goto Y., Suga H., Murakami H. Reevaluation of the D-amino acid compatibility with the elongation event in translation. Journal of the American Chemical Society. 2013;135(5):1830-1837. https://doi.org/10.1021/ja309570x
10. Yamashita M., Ogasawara M., Kawasaki Y., Niisato M., Saito H. [et al.]. Deficiency of protein-L-isoaspartate (D-aspartate) O-methyl-transferase expression under endoplasmic reticulum stress promotes epithelial mesenchymal transition in lung adenocarcinoma. Oncotarget. 2018;9(17):13287-13300. https://doi.org/10.18632/oncotarget.24324

Keywords: D-asparagine, dermal fibroblast, proliferative activity, human

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy