Medical news
of the North Caucasus
Scientific journal
Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137

Site search

Correspondence address
310 Mira Street, Stavropol, Russia, 355017

+7 8652 352524; +7 8652 353229.

+7 8652 352524.


The frequency of polymorphism –262 C>T CAT gene of infertile men in the moscow region

Galina Myandina; Nina Kulchenko; Hasan Alhejoj;

Follow up, 138 men of reproductive age of the Moscow region were performed. The standard clinical examination with double spermogram analysis and determination of reactive oxygen species in the ejaculate was produced. Using the polymerase chain reaction of DNA synthesis, we examined DNA samples of blood leukocytes for the presence of –262 C>T polymorphisms of the CAT gene. The semen analysis of 70 (50.7 %) patients showed pathospermia (main group). In 68 (49.3 %) men, we discovered normozoospermia (control group). In 35 (50.0 %) patients with pathospermia identified local mutations of the gene CAT. Also, 2/3 of the participants were registered with the heterozygous genotype (262СТ), and 7(10 %) men were marked by the genotype homozygous for the defective allele (262ТТ). ed local mutations of the CAT gene in both heterozygous and homozygous types. We defined a direct correlation between
the level of reactive oxygen species and the frequency of CAT gene polymorphisms –262 C>T in infertile men (r=0.7). In conclusion, the CAT C–262T (rs1001179) gene polymorphism occurs in 50 % of cases in infertile men of Moscow region. Allele –262t of the СAT catalase gene can be considered as a genetic risk factor for oxidative stress and pathospermia.


1. Vinogradov I. V., Zhivulko A. R., Vinogradova L. M., Korolev S. V. Docosahexaenoic acid in the treatment of male infertility. Andrology and Genital Surgery. 2018;19(4):21-27. https://doi.org/10.17650/2070-9781-2018-19-4-21-27
2. Agarwal A., Virk G., Ong C., du Plessis S. S. Effect of oxidative stress on male reproduction. The World Journal of Men’s Health. 2014;32:1-17.
3. Kulchenko N. G. Oxidative stress in the development of non-obstructive azoospermia. Difficult patient. 2017;15(4-5):44-46.
4. Aitken R. J., Baker M. A., De Iuliis G. N., Nixon B. New insights into sperm physiology and pathology. Handb. Exp. Pharmacol. 2010;(198):99-115.
5. Rodriguez A. G., de la Casa M., Johnston S., Gosalvez J., Roy R. Association of polymorphisms in genes coding for antioxidant enzymes and human male infertility. Annals of Human Genetics. 2019;83(1):63-72. https://doi.org/10.1111/ahg.12286
6. Kulchenko N. G., Myandina G. I., Alhedjoj H. Association-genetic study of polymorphism G-105A SEPS1 gene in male infertility. Research and Practical Medicine Journal. 2018;5(2):65-71. https://doi.org/10.17709/2409-2231-2018-5-2-7
7. Kaprin A. D., Kostin A. A., Kulchenko N. G., Fomin D. K.,Aliev A. R. O. Diagnosis of idiopathic infertility. What’s new? Vestnik Rossijskogo nauchnogo centra rentgenoradiologii Minzdrava Rossii. 2014;2:3.
8. Sabouhi S., Salehi Z., Bahadori M. H., Mahdavi M. Human catalase gene polymorphism (CAT C-262T) and risk of male infertility. Andrologia. 2014;47:97–101.
9. Saify K. Genetic polymorphisms in the promoter region of catalase gene, creates new potential PAX-6 and STAT4 response elements. Mol. Biol. Res. Commun. 2016;5(2):97-100.
10. Tefik T., Kucukgergin C., Sanli O., Oktar T., Seckin S. [et al.] Manganese superoxide dismutase Ile58Thr, catalase C–262T and myeloperoxidase G-463A gene polymor phisms in patients with prostate cancer: relation to advanced and metastatic disease. BJU Int. 2013;112(4):406-414.
11. Sousa V. C., Carmo R. F., Vasconcelos L. R., Aroucha D. C., Pereira L. M. [et al.] Association of Catalase and Glutathione Peroxidase 1 Polymorphisms with Chronic Hepatitis C Outcome. Ann. Hum. Genet. 2016;80(3):145-53. https://doi.org/10.1111/ahg.12152

Keywords: male infertility, oxidative stress, CAT gene, polymorphism, pathospermia

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy