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The frequency of polymorphism –262 C>T CAT gene of infertile men in the moscow region

[Urology]
Galina Myandina; Nina Kulchenko; Hasan Alhejoj;

Follow up, 138 men of reproductive age of the Moscow region were performed. The standard clinical examination with double spermogram analysis and determination of reactive oxygen species in the ejaculate was produced. Using the polymerase chain reaction of DNA synthesis, we examined DNA samples of blood leukocytes for the presence of –262 C>T polymorphisms of the CAT gene. The semen analysis of 70 (50.7 %) patients showed pathospermia (main group). In 68 (49.3 %) men, we discovered normozoospermia (control group). In 35 (50.0 %) patients with pathospermia identified local mutations of the gene CAT. Also, 2/3 of the participants were registered with the heterozygous genotype (262СТ), and 7(10 %) men were marked by the genotype homozygous for the defective allele (262ТТ). ed local mutations of the CAT gene in both heterozygous and homozygous types. We defined a direct correlation between
the level of reactive oxygen species and the frequency of CAT gene polymorphisms –262 C>T in infertile men (r=0.7). In conclusion, the CAT C–262T (rs1001179) gene polymorphism occurs in 50 % of cases in infertile men of Moscow region. Allele –262t of the СAT catalase gene can be considered as a genetic risk factor for oxidative stress and pathospermia.

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Keywords: male infertility, oxidative stress, CAT gene, polymorphism, pathospermia


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