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Series ПИ #ФС 77-26521.
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ISSN 2073-8137

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Quantifying analysis of advanced glycosylation end products (ages) expression in periodontitis patie ts with diabetes type II

[Internal diseases]
Wolf-Dieter Grimm; Evgeny Shchetinin; Dmitry Bobryshev; Sergey Sirak;

Diabetes is an established risk factor for periodontitis. we investigated AGEs distribution in human gingival tissues and monocyte cytokine expression in diabetic patients with chronic periodontitis (DII/cP). Within indicated surgical procedures gingival biopsies were taken from 10 DII/cP and from 10 healthy controls. Tissue samples were prepared for immunohistochemistry using a monoclonal antibody specific for AGEs (6D12, TransGenic Inc.) and IMAGES J was used for quantification. Mean clinical parameters in DII/cP presented typical for severe chronic periodontitis (mt: 10.5; BoP: 84%; PBI: 2.9; cAL: 5.5 mm; rAL: 6.9 mm). In gingival tissue from DII/cP immunohistochemistry showed enhanced accumulation of AGEs (17%) compared to controls (13%). This study found that diabetes and periodontitis results in further AGE-accumulation compared to periodontitis alone. These results suggest the possibility that the blockade of RAGE may provide an effective approach in a range of diabetic complications including periodontal disease.


1. Brett J., Schmidt A. M., Yan S. D., Zou Y. S., Weidman E., Pinsky D., Nowygrod R., Neeper M., Przysiecki C., Shaw A. et al. Am J Pathol 1993;143:1699-1712.
2. Brownlee M. Lilly Lecture 1993. Diabetes 1994;43:836-841.
3. Brownlee M. Annu Rev Med 1995;46:223-234.
4. Brownlee M., Cerami A., Vlassara H. N Engl J Med 1988;318:1315-1321.
5. Chapple I. L. J Clin Periodontol 1997;24:287-296.
6. Charonis A. S., Reger L. A., Dege J. E., Kouzi-Koliakos K., Furcht L. T., Wohlhueter R.M., Tsilibary E.C. Diabetes 1990;39:807-814.
7. Chang P. C., Tsai S. C., Chong L. Y., Kao M. J. J Periodontol 2014;85;e268-e276 , doi:10.1902/jop.2014a.130554)
8. Chang P. C., Tsai S. C., Jheng Y. H., Lin Y. F., Chen C. C. J Dent Res 2014;93(4):388-93. doi:10.1177/0022034514523785. Epub 2014 Feb 19.
9. Engebretson S.P., Hey-Hadavi J., Ehrhardt F.J., Hsu D., Celenti R.S., Grbic J.T., Lamster I.B. J Periodontol 2004;75:1203-1208.
10. Garg A. Diabetes 1992;41 Suppl 2:111-115.
11. Gassmann G., Kaltschmidt B., Kaltschmidt C., Grimm W. D. Dtsche Zahnärztebl 2006;3:83-90.
12. Grimm W. D., Meiselbach V., Gassmann G. Dtsche Zahnärztebl 2006;1+2:10-16.
13. Grimm W.D., Petrie J., Philippou S. Advanced glycated endproducts accelerating periodontal disease associated with diabetes. http://iadr.confex.com/ iadr/2004Hawaii/techprogram/abstract_39949.htm 2004.
14. Grimm W. D., Southerland J.H. Parodontologie 2004;15:280.
15. Hassar H., Kantarci A., van Dyke Th.E. Periodontol 2000 2007;43:233–244. doi:10.1111/j.1600-0757.2006.00168.x
16. Holla L. I., Kankova K., Fassmann A., Buckova D., Halabala T., Znojil V., Vanek J. J Periodontol 2001;72:1742-1746.
17. Hugoson A., Thorstensson H., Falk H., Kuylenstierna J.
J Clin Periodontol 1989;16:215-223.
18. John W.G., Lamb E.J. Eye 1993;7 (Pt 2):230-237.
19. Katz J., Bhattacharyya I., Farkhondeh-Kish F., Perez F. M., Caudle R.M., J Clin Periodontol 2005;32:40-44.
20. Lalla E., Cheng B., Lal S., Tucker S., Greenberg E., Goland R., Lamster I. B. Diabetes Care 2006;29:295-299.
21. Lalla E., Kaplan S., Chang S. J., Roth G. A., Celenti R., Hinckley K., Greenberg E., Papapanou P.N. J Clin Periodontol 2006;33:855-862.
22. Lalla E., Lamster I. B., Feit M., Huang L., Spessot A., Qu W., Kislinger T., Lu. Y., Stern D. M., Schmidt A. M. J Clin Invest 2000;105:1117-1124.
23. Lalla E., Lamster I. B., Hofmann M.A., Bucciarelli L., Jerud A. P., Tucker S., Lu. Y., Papapanou P.N., Schmidt A.M. Arterioscler Thromb Vasc Biol 2003;23:1405-1411.
24. Ligier S., Fortin P.R., Newkirk M.M. Br J Rheumatol 1998;37:1307-1314.
25. Mealey B. Diabetes and periodontal diseases. J Periodontol 1999;70:935-949.
26. Mealey B. L., Oates T. W. J Periodontol 2006;77:1289-1303.
27. Monnier V.M. J Gerontol 1990;45:B105-111.
28. Monnier V.M., Glomb M., Elgawish A., Sell D.R. Diabetes 1996;45 Suppl 3:S67-72.
29. Morimoto-Yamashita Y., Ito T., Kawahara K., Kikuchi K., Tatsuyama-Nagayama S., Kawakami-Morizono Y., Fijisawa M., Miyashita K., Emoto M., Torii M., Tokuda M. Med Hypotheses. 2012 Oct;79(4):452-5. doi:10.1016/j.mehy.2012.06.020.
30. Nakamura Y., Horii Y., Nishino T., Shiiki H., Sakaguchi Y., Kagoshima T., Dohi K., Makita Z., Vlassara H., Bucala R. Am J Pathol 1993;143:1649-1656.
31. Neeper M., Schmidt A. M., Brett J., Yan S. D., Wang F., Pan Y. C., Elliston K., Stern D., Shaw A. J Biol Chem 1992;267:14998-15004.
32. Pahl H. L. Oncogene. 1999;18:6853-6866.
33. Schmidt A. M., Hori O., Cao R., Yan S. D., Brett J., Wautier J. L., Ogawa S., Kuwabara K., Matsumoto M., Stern D. Diabetes. 1996;45 Suppl 3:S77-80.
34. Schmidt A. M., Weidman E., Lalla E., Yan S. D., Hori O., Cao R., Brett J. G., Lamster I. B. J Periodontal Res 1996;31:508-515.
35. Sirak S. V., Rezepova G. T. Stomatologiya. 2006;85(2):31-33. (In Russ).
36. Stamler J. Epidemiology, established major risk factor and the primary prevention of coronary heart disease. Philadelphia:1987
37. Tsilibary E. C., Charonis A. S., Reger L. A., Wohlhueter R. M., Furcht L. T. J Biol Chem 1988;263:4302-4308.
38. Wautier J. L., Guillausseau P. J. Diabetes Metab 2001;27:535-542.

Keywords: diabetes, periodontitis, advanced glycosylation end products, immunohistochemistry

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy