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ISSN 2073-8137
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PEP-36E and ABP-9L cationic peptides stability evaluation in blood serum and under thermal stress in vitro

[Abstracts]
Albert Bolatchiev; Albert Ashotovich Vartanyan;

The study aimed to evaluate the impact that blood serum and heating have on the in vitro antibacterial activity of the PEP-36E and ABP-9L cationic peptides. The activity was identified through measuring the minimum inhibitory concentration (MIC) using the serial dilution method against a clinical isolate of Escherichia coli. The control group had the MIC values at 12.5 and 10 μg/mL, respectively. Following a 24-hour incubation period with serum (1:1, 37 ºC), the activity of both peptides remained undetectable (MIC>64 μg/mL), which points at their degradation by serum peptidases. Heating the samples at 70–90 ºC for 30–60 minutes revealed no effect on their activity. Given this, while these peptides demonstrate significant thermostability, they remain unstable in serum, which is to be taken into account when developing both systemic and local dosage forms.

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Keywords: antibiotic resistance; antimicrobial peptide; blood serum; thermal stability; de novo peptide development


Founders:
Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy