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https://doi.org/10.14300/mnnc.2024.19067

Netosis-forming ability of neutrophils in children with respiratory tuberculosis

[Pediatrics]
Anna Mordyk; Dmitry Georgyevich Novikov; Alexander Nikolaevich Zolotov; Maria Romanova; Nikolay Aleksandrovich Kirichenko; Alexander Olegovich Ptukhin;

The aim of the study was to evaluate the NETosis-forming ability of peripheral blood neutrophils in children with respiratory tuberculosis.

Using fluorescent microscopy, the percentage of intact neutrophils, activated neutrophils, early netosis cells, cloud shaped NETs, and filamentous NETs was calculated.

Neutrophils in the study group formed cloud-shaped NETs significantly more often (p=0.0007). The median proportion of this type of trap in the control group (with a negative reaction to tuberculin) was 4.9 %, and in the group with tuberculosis – 15.7 %. The proportion of cloud-shaped NETs in the tuberculosis group was higher than the proportion of filamentous NETs. Thus, the neutrophils of children with TB formed mainly cloudy NETs, which is not characteristic of healthy children, whose neutrophils more often were formed by filamentous NETs.

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Keywords: Tuberculosis, children, neutrophil extracellular traps, NETosis, neutrophil’s NETs-forming ability