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https://doi.org/10.14300/mnnc.2020.15122

MiRNK expression in patients with malignant and precancerous cervical diseases

[Oncology]
Oleg Kit; Maria Timoshkova; Alexey Maksimov; Ekaterina Verenikina; Madinat Mazhitovna Kecherukova; Ekaterina Lukyanova;

The purpose of this study was an analysis of expression profiles of miRNA-20a, miRNA-21, miRNA-143, miRNA-23b, and miRNA-218 in patients with malignant and precancerous cervical diseases. The study included 145 patients with T1а1-T2a1N0M0 of cervical cancer (CC) and 137 patients with histologically verified squamous intraepithelial lesions of the cervix observed. Expression of five miRNA markers – miRNA-20a, miRNA-21, miRNA-143, miRNA-23b, and miRNA-218 was evaluated in tissues of the tumor, its perifocal area (1 cm from the visible tumor edge) and in visually unchanged tissues obtained during surgery. The cervical epithelium of CC patients demonstrated a sharp increase in the expression of pro-tumor miRNA-20a and miRNA-21, a pronounced decrease in anti-tumor miRNA-23b, and a tendency to declining expression of miRNA-143 and miRNA-218. Two subgroups of patients – with CC and high-grade SIL – clearly differed in miRNA-20a levels, which was highly significant for the differential diagnosis of the two conditions. Expression of miRNA-21 and miRNA-23b in the cervical epithelium was high in both CC and high-grade SIL, which limited their potential in differentiation between cancerous and precancerous changes in the cervix.

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Keywords: miRNA-20a, miRNA-21, miRNA-23b, miRNA-143, miRNA-218, expression, cervical cancer (CC), squamous intraepithelial lesion (SIL)