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Clinical -morphological and immunohistochemical study of tissues during periimplantit and periodontitis

Anatoly Alekseevich Kulakov; Evgenia Kogan; Vladimir Nikolenko; Tatyana Vladislavovna Brailovskaya; Anna Petrovna Vedyaeva; Ekaterina Valeryevna Blinova;

A comparative clinical-morphological and immunohistochemical study of gum tissue with severe inflammatory
complications in the area of dental implants and teeth in patients with chronic generalized periodontitis was carried out. We studied 50 biopsy samples from 27 patients (14 women and 13 men) aged 35–60 years with diagnoses of peri-implantation mucositis (7 patients), peri-implantitis (14 patients), severe generalized periodontitis (6 patients). Immunohistochemical reactions with antibodies to Ki-67 (at a dilution of 1:100, Dako), VEGF (at a dilution of 1:100, Abcam), SMA (LabVision, at a concentration of 1:100) were performed on serial paraffin sections. It is established that when comparing chronic generalized periodontitis of severe degree and peri-implantitis, the latter is characterized by more pronounced inflammatory-destructive process in the implant area, caused by the addition of immune inflammation with the formation of a fibrous capsule around the implant. According to the data of immunohistochemical differences in SMA, VEGF, Ki-67, peri-implantation mucositis and peri-implantitis are successive stages of progression of one process.


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Keywords: peri-implantation mucositis, peri-implantitis, growth zones, SMA, Ki-67, VEGF

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy