Medical news
of the North Caucasus
Scientific journal
Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137

Site search

Correspondence address
310 Mira Street, Stavropol, Russia, 355017

+7 8652 352524; +7 8652 353229.

+7 8652 352524.


Morphofunctional changes in the liver and the state of the rat blood coagulation system in development of reperfusion syndrome

[Experimental medicine]
Anatolii Arkadevich Pisarev; Lyudmila Vasilievna Anisimova; Irina Ivanovna Fomochkina; Anatoly Vladimirovich Kubyshkin; Dmitry Sergeevich Kuzichkin; Yulianna Ivanovna Shramko; Vladimir Zakharovich Kharchenko; Elena Petrovna Golubinskaya;

Disseminated intravascular coagulation leads to the development of multiple organ failure syndromes with a mortality rate higher than 50 %. We examined the pathogenetic mechanisms of formation of systemic reperfusion syndrome in a rat model of reperfusion syndrome for 6, 12, and 24 hours. A total of 60 white Wistar rats, with a weight of 180–200 g, were studied. We found that morphological changes in the liver were manifested by formation of triggers for hepatocyte damage, including activation of apoptosis and initiation of pro-inflammatory damage pathways. These changes did not lead to severe alterations in functional activity of the liver. Disturbances in the blood coagulation system that developed with reperfusion syndrome with a potential threat of developing DIC were associated with increased consumption of coagulation factors. These disturbances in the blood coagulation system were also associated with inhibition of synthetic function of the liver to a lesser extent.


1. Intagliata N. M., Davis J.-P. E., Caldwell S. H. Coagulation Pathways, Hemostasis, and Thrombosis in Liver Failure. Seminars in respiratory and critical care medicine. 2018;39(5):598-608. https://doi.org/10.1055/s-0038-1673658
2. Aithal G. P., Watkins P. B., Andrade R. J. Case definition and phenotype standardization in drug‐induced liver injury. Clinical Pharmacology & Therapeutics. 2011;89(6):806-815. https://doi.org/10.1038/clpt.2011.58
3. Morozov Y. A., Mednikov R. V., Charnaya M. A. Hemostasis disorders and their diagnosis in liver pathology. Gemorragicheskie diatezy, trombozy, trombofilii. – Hemorrhagic diathesis, thrombosis, thrombophilia. 2014;(1):162-171.
4. Batyrova A. S., Bakanov M. I., Surkov A. N. Modern concepts of the hemostasis system in chronic liver diseases (literature review). Klinicheskaya laboratornaya diagnostika. – Clinical laboratory diagnosis. 2015;60(8):40-44.
5. Kurkina I. A., Maevskaya M. V., Ivashkin V. T. Hypercoagulation and thrombosis in patients with liver cirrhosis. Poliklinika. – Polyclinic. 2015;4(2):7-10.
6. Gando S., Levi M., Toh C. H. Disseminated intravascular coagulation. Nature Reviews Disease Primers. 2016;2:1603-1607. https://doi.org/10.1038/nrdp.2016.37
7. Cohen M. J., Call M., Nelson M., Calfee C. S., Esmon C. T. [et al.] Critical role of activated protein C in early coagulopathy and later organ failure, infection and death in trauma patients. Annals of surgery. 2012;255(2):379-385. https://doi.org/10.1097/SLA.0b013e318235d9e6
8. Kubyshkin A. V., Pylayeva N. Yu., Fomochkina I. I., Pisarev A. A. Trauma, hemostasis and disseminated intravascular coagulation syndrome: pathogenic mechanisms of coagulation disorders. Klinicheskaya patofiziologiya. –Clinical pathophysiology. 2016;22(4):104-117.
9. Litvitskii P. F. Pathology of the hemostatic system. Voprosy sovremennoy pediatrii. – Current Pediatrics. 2014;13(2):65-76. https://doi.org/10.15690/vsp.v13i2.974
10. Thachil J. The Elusive Diagnosis of Disseminated Intravascular Coagulation: Does a Diagnosis of DIC Exist Anymore? Semin. Thromb. Hemost. 2019;45(1):100-107. https://doi.org/10.1055/s-0038-1677042
11. Yoshimura J., Yamakawa K., Kodate A., Kodate M., Fujimi S. Prognostic accuracy of different disseminated intravascular coagulation criteria in critically ill adult patients: a protocol for a systematic review and meta-analysis. BMJ Open. 2018;8(12):e024878. https://doi.org/10.1136/bmjopen-2018-024878
12. Levi M., Toh C. H., Thachil J., Watson H. G. Guidelines for the diagnosis and management of disseminated intravascular coagulation. British Committee for Standards in Haematology.British journal of haematology. 2009;145(1):24-33. https://doi.org/10.1111/j.1365-2141.2009.07600.x
13. Carden D. L., Granger D. N. Pathophysiology of ischemia-reperfusion injury. The Journal of Pathology. 2000;190(3):255-266. https://doi.org/10.1002/(SICI)1096-9896(200002)190:33.0.CO;2-6
14. Hughes S. F., Hendricks B. D., Edwards D. R., Bastawrous S. S., Roberts G. E. [et al.] Mild episodes of tourniquet- induced forearm ischemia-reperfusion injury results in leukocyte activation and changes in inflammatory and coagulation markers. Journal of inflammation. 2007;4:12. https://doi.org/10.1186/1476-9255-4-12

Keywords: liver, blood coagulation system, reperfusion syndrome, apoptosis, inflammation

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy