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EBSCO

https://doi.org/10.14300/mnnc.2019.14106

Content of B- and T-cells in the spleen of patients with aplastic anemia

[Original research]
Nadezhda Stanislavovna Fedorovskaya; Semen Venediktovich Petrov; Valery Borisovich, Zaitsev;

Using histological, immunohistochemical (IHC) and morphometric methods, B- and T-cells, we studied the spleens of 30 patients with aplastic anemia (AA). The median age is 21.0 (18.5; 38.5) years. The control group consisted of 20 persons who died suddenly and who did not have a history of blood diseases. In the histological material of the spleens, with IHC staining and morphometry, the relative numerical values of B-cells and T-cells populations in the functional areas of the organ in AA patients were obtained. Recalculation of morphometric parameters in absolute values (g) taking into account the mass of the spleen was carried out according to the original methodology. Evaluation of the content of B- and T-cells in the spleen made it possible to determine the factors predicting the course of development of AA after splenectomy. Signs which determine the unfavorable prognosis for the progression of disease are as follows: the absolute number of CD4+ T-cells in the spleen is less than 5.4 g and the Mass Ratio Index (MRI) CD4+ / CD8+ T-cells is less than or equal to 1.0. Consequentially that justifies a need for an individual approach to further immunosuppressive therapy in this category of patients.

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Keywords: human spleen, B-cells, T-cells, aplastic anemia