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Changes in the membrane potential of mitochondria in dependence on the continuance of ischemia and reperfusion of liver in rats

[Original research] [Experimental medicine]
Konstantin Popov; Ilya Bykov; Igor Tsymbalyuk; Alexander Petrovich Storozhuk; Anatoly Nikolaevich Kurzanov; Elena Evgenevna Esaulenko;

The study in the membrane potential of hepatocyte mitochondria in dependence on the various continuances of the ischemic and the following reperfusion period has been performed. The received data has demonstrated the gradual decrease in the membrane potential of mitochondria extracted from the liver tissue of rats after the ischemic periods of various continuances. The decrease in this index has practically reached the null-values after 25-minute clamping of the analogue of hepatoduodenal ligamentum. At the same time the tendency to the restoration of normal values for mitochondrial membrane potential at the reperfusion period has been revealed, most typically for the liver tissue after 10–15 minute ischemia (by 30–60 %, up to 102–154 mv). Thus, the received data demonstrate the high lability of the functional state of mitochondria at the ischemic-reperfusion disorder of hepatocytes and can be used for studies in the course peculiarities for various hypoenergetical conditions as well as for evaluation of effectiveness of mitochondrial cytoprotectors within the experimental correction of wide range of pathological processes.

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References:
1. Danilenko L. M., Pokrovsky M. V., Tatarenkova I. A., Elagin V. V., Bratchikov O. I.
Pharmacological preconditioning of resveratrol in ischemic / reherfusion injury: the role of nitric oxide. Kubanskij nauchnyj medicinskij vestnik. – Kuban scientific medical journal. 2015;155(6):35-38. (In Russ.).
2. Tsymbalyuk I. Y., Manuilov A. M., Popov K. A., Basov A. A. Metabolicheskaya
korrektsiya dikhloratsetatom natriya ishemicheskireperfuzionnogo povrezhdeniya pri sosudistoy izolyatsii pecheni v eksperimente. Novosti Khirurgii. Surgery News. 2017; 25(5):447-453. (In Russ.).
3. Zvyagina V. I., Belskikh E. S., Uryasev O. M., Medvedev D. V., Kiseleva V. A. [et al.]. Vliyaniye karnitina khlorida na mitokhondrii serdtsa krys pri modelirovanii gipergomotsisteinemii. Meditsinskii vestnik Severnogo Kavkaza. – Medical News of North Caucasus. 2018;13(1-1):78-81. (In Russ.). https://doi.org/10.14300/mnnc.2018.13022
4. Zorova L. D., Popkov V. A., Plotnikov E. J., Silachev D. N., Pevzner I. B. [et al.]. Funktsionalnaya znachimost mitokhondrialnogo membrannogo potentsiala. Biologicheskiye membrany. Zhurnal membrannoj i kletochnoj biologii. Membrane and Cell Biology J. 2017;34(6):93-100. (In Russ.). https://doi.org/10.7868/S0233475517060020
5. Ionov D. I., Fedorova M. M., Galenko-Yaroshevsky P. A., Kezeli T. D., Mirziashvili M. A. [et al.]. Vliyaniye kardiotonicheskikh sredstv razlichnoy prirody na bioenergeticheskiye protsessy v miokarde pri sochetanii sindroma sistemnogo vospalitel’nogo otveta i serdechnoy nedostatochnosti. Kubanskij nauchnyj medicinskij vestnik. – Kuban scientific medical journal. 2016;156(1):52-56. (In Russ.).
6. Dzhimak S. S., Basov A. A., Volchenko N. N., Samkov A. A., Baryshev M. G. [et al.]. Changes in the functional activity of mitochondria isolated from the liver of rat that passed the preadaptation to ultra-low deuterium concentration. Doklady Biochemistry and Biophysics.2017;476(1):323-325. https://doi.org/10.1134/S1607672917050088
7. Panov A. V. Practical mitochondriology: pitfalls and problems in studies of mitochondria with a description of mitochondrial functions. Charlston, NC USA: Create Space; 2015.
8. Perevoshchikova I. V., Sorochkina A. I ., Zorov D. B., Antonenko Y. N. Safranin O kak fluorestsentnyy indicator membrannogo potentsiala mitokhondriy: issledovaniye na urovne suspenzii i urovne otdelnykh mitokhondriy. Biokhimiya. – Biochemistry. 2009;74(6): 663-671.https://doi.org/10.1134/S000629790906011X
9. Cheshchevik V. T. Mitochondrial membrane potential of rat hepatocytes under toxic injury. Izvestiya Nacional’noj akademii nauk Belarusi. Seriya biologicheskih nauk. – Proceedings of the National Academy of Sciences of Belarus, Biological Series. 2010;2:75-80. (In Russ.).
10. Bunting J. R., Phan T. V., Kamali E., Dowben R. M. Fluorescent cationic probes of mitochondria. Metrics and mechanism of interaction. Biophysical Journal. 1989;56(5):979-993. https://doi.org/10.1016/S0006-3495(89)82743-2
11. Lin H. C., Liu S. Y., Lai H. S., Lai I. R. Isolated mitochondria infusion mitigates ischemia reperfusion injury of the liver in rats. Shock. 2013;39(3):304-310. https://doi.org/10.1097/SHK.0b013e318283035f
12. McCully J. D., Levitsky S., del Nido P. J., Cowan D. B. Mitochondrial transplantation for therapeutic use. Clinical and Translational Medicine. 2016;5:16. https://doi.org/10.1186/s40169-016-0095-4

Keywords: mitochondria, membrane potential, safranin, ischemia, reperfusion, liver