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ISSN 2073-8137
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Bone marrow and adipose-derived rat mesenchymal stem cells for recellularization of biological matrices

[Original research] [Experimental medicine]
Еlena Kuevda; Elena Gubareva; Ivan Gumenyuk; Ramazan Nakohov; Dmitry Petrovich Puzanov;

The morphological features, proliferative activity and flexibility of rat mesenchymal multipotent stem cells (MSC) bonemarrow derived and isolated from the adipose tissue stromal-vascular fraction were evaluated. MMSCs were verified by the negative CD 34 and the positive CD 90, and by induced differentiation. Cells proliferative activity was evaluated by Ki-67 cytochemical staining with qualitative measurement of the luminescence intensity. XTT reagent was used to study the comparative cell populations’ viability on decellularized rat oesophageal and diaphragm matrices, obtained by detergent-enzymatic method. The cytotoxic properties of the scaffolds and cell lines viability at recellularization of decellularized rat esophagus and diaphragm matrices were evaluated by quantitating the percentage of metabolically active cells. Taking into account the obtained data, the most optimal cell fraction for biological scaffolds recellularization was selected.

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Keywords: mesenchymal stem cells, biological scaffolds, cytotoxicity, adipose tissue, bone marrow


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Pyatigorsk State Research Institute of Balneotherapeutics
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