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ISSN 2073-8137
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Optimization of the decellularization protocol with the purpose of preserving the angiogenic properties of the biological scaffold

[Original research] [Experimental medicine]
Elena Gubareva; Еlena Kuevda; Mikhail Bykov; Ramazan Nakohov; Ivan Pavlyuchenko; Oleg Tsymbalov; Tatiana Gayvoronskaya; Oleg Tereshchenko;

In the framework of the experimental study, the esophagus decellularization protocol was optimized in small laboratory animals, the morphological structure of the resulting acellular matrix was studied, the quantitative evaluation of residual DNA was made, the acellular scaffold was recellularized with mesenchymalmultipotent stem cells to assess cytotoxicity, maintain cell viability and metabolic activity, and heterotopic and orthotopic transplantations of decellularized and recellularized matrices in rats were performed. It is shown that the resulting decellularized esophagus matrix preserves the architectonics of native tissue with complete removal of cellular material, does not possess cytotoxic properties, supports adhesion and cell proliferation, and has proangiogenic properties, including preserved components of the extracellular matrix, which could take an active part in neoangiogenesisand stimulate microvascular proliferation of endotheliocytes in hetero- and orthotopic transplantation. All the facts allow us to consider the developed and modified protocol of esophageal decellularization to be a promising way to obtain a biological scaffold with pro-angiogenic properties for creating tissue engineered constructions and to give the possibility of clinical application in the foreseeable future.

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Keywords: tissue engineered constructions, esophagus, decellularized matrix, angiogenesis


Founders:
Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy