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https://doi.org/10.14300/mnnc.2019.14005

Treatment of infected wounds with a chitosan-collagen complex with dioxydin and lidocain in the experimental conditions

[Original research] [Experimental medicine]
Alexander Bezhin; Vyacheslav Lipatov; Arsen Grigoryan; Edward Fronchek; Muhammad David Naimzada;

This article presents the results of experimental testing of chitosan-collagen wound dressing with dioxydin and lidocain when healing the infected wounds. The experiment was performed on Wistar rats with a modeled infected wound. The treatment was carried out daily for 15 days. The results are evaluated on the basis of planimetric, bacteriological, histological, morphometric, biochemical and hematological methods of research, the data were processed statistically. It was found that by the 15th day the wounds had decreased by more than 98 %, the maximum healing rate was at 1–5 days. Leukocytosis with lymphocytosis, noted on the days 1 and 5, disappeared by the 10th day of the experiment. Wound contamination at the beginning of the experiment was 34.4x106 CFU/g, by 15 days it decreased to 1.7x104 CFU/g. The histological and morphometric data confirmed that by 15 days the exudation phase was fully completed and the peak of the proliferative phase was passed. Indicators of hydroxyproline test showed an increase in its concentration, which indicates the active synthesis of collagen in the area of the defect.

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Keywords: wound treatment, wound process, chitosan, collagen, dioxydin, lidocain, wound covering, infected wound