Medical news
of the North Caucasus
Scientific journal
Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137

Site search

Correspondence address
310 Mira Street, Stavropol, Russia, 355017

+7 8652 352524; +7 8652 353229.

+7 8652 352524.


Changes in state of the thiol linkages of an antioxidant system during ischemia and reperfusion, against a background of vascular exclusion in the rat liver

[Experimental medicine]
Konstantin Popov; Ilya Bykov; Igor Tsymbalyuk; Mikhail Bykov;

The dynamic activities of glutathione reductase (GR) and glutathione peroxidase (GPO), as well as the concentration of reduced glutathione (GSH), were examined at both the organ and systemic levels in a liver ischemia-reperfusion model. Samples were collected at 5 min intervals from rats (n=95) during a 20 min ischemic period and after 20 min reperfusion. There was an adaptive increase (30–50 %) in GR activity and GSH concentration in erythrocytes during the ischemic period. Within 10 min of ischemia, there was a 1.5- to 2-fold decrease in all parameters measured in the liver tissue. Liver reperfusion was accompanied by greater decreases in GR and GPO activities, as well as increased GSH concentrations, in the erythrocytes. In liver tissue, against the background of increased oxygen toxicity, there was a 2- to 3-fold increase in GPO activity. GSH concentration and the GR activity remain decreased during 10 min reperfusion but then returned to control levels. These findings provide information about the pathogenesis of ischemic reperfusion and also illustrate pathobiochemical links that are potentially subject to beneficial metabolic correction.


1. Novomlinsky V. V. Studying metabolic and morphohistochemical changes of ischemized liver after reperfusion. Vestnik ehksperimentаl’noj i klinicheskoj khirurgii. – Journal of Experimental and Clinical Surgery. 2009;2(1):44-50.
2. Tsymbalyuk I. Y., Manuilov A. M., Popov K. A., Basov A. A. Metabolic correction of the ischemia-reperfusive injury with sodium dichloroacetate in vascular isolation of the liver in experiment. Novosti Khirurgii. 2017;25(5):447-453.
3. Dzhimak S. S., Basov A. A., Volchenko N. N., Samkov A. A., Baryshev M. G., Fedulova L. V. Changes in the functional activity of mitochondria isolated from the liver of rat that passed the preadaptation to ultra-low deuterium concentration. Doklady Biochemistry and Biophysics. 2017. 476(1):323-325. https://doi.org/10.1134/S1607672917050088
4. Vardanian A. J., Busuttil R. W., Kupiec-Weglinski J. W. Molecular mediators of liver ischemia and reperfusion injury: a brief review. Mol. Med. 2008;14:337-345. https://doi.org/10.2119/2007-00134
5. Bykov M. I., Basov A. A. Change of parameters in prooxidant-antioxidant bile system in patients with the obstruction of bile-excreting ducts. Medical News of North Caucasus. 2015;10(2):131-135. https://doi.org/10.14300/mnnc.2015.10029
6. Alehin S. A., Pokrovsky M. V., Ivanova L. V., Nazarenko D. P., Kolmikov D. I., Buslovskaia L. K., Kolesnik I. M., Pisarev D. I. Modulation of intra-abdominal organs microvessels reaction for ischemia/reperfusion by recombinant erythropoietin. Kubanskij nauchnyj medicinskij vestnik. – Kuban Scientific Medical Journal. 2014;144(2):9-11.
7. Danilenko L. M., Pokrovsky M. V., Tatarenkova I. A., Elagin V. V., Bratchikov O. I. Pharmacological preconditioning of resveratrol in ischemic/ reperfusion injury: the role of nitric oxide. Kubanskij nauchnyj medicinskij vestnik. – Kuban Scientific Medical Journal. 2015;155(6):35-38.
8. Nastos C., Kalimeris K., Papoutsidakis N., Tasoulis M. K., Lykoudis P. M., Theodoraki K., Nastou D., Smyrniotis V., Arkadopoulos N. Global consequences of liver ischemia/ reperfusion injury. Oxid. Med. Cell. Longev. 2014;1-13. https://doi.org/10.1155/2014/906965
9. Karpishchenko A. I. Handbook. Medical Laboratory Technology. Sankt-Petersburg: Intermedika, 2002.
10. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem. 1976;72:248-254. https://doi.org/10.1016/0003-2697(76)90527-3
11. Dominko K., Đikić D. Glutathionylation: a regulatory role of glutathione in physiological processes. Arh. Hig. Rada. Toksikol. 2018;69:1-24. https://doi.org/10.2478/aiht-2018-69-2966
12. Kulinsky V. I., Kolesnichenko L. S. The glutathione system. II. Other enzymes, thiol-disulfide metabolism, inflammation, and immunity, functions. Biochemistry (Moscow) Supplement. Series B: Biomedical chemistry. 2009;3(3):211-220. https://doi.org/10.1134/S1990750809030019

Keywords: ischemia-reperfusion syndrome, liver, antioxidant system, glutathione

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy