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Changes in state of the thiol linkages of an antioxidant system during ischemia and reperfusion, against a background of vascular exclusion in the rat liver

[Experimental medicine]
Konstantin Popov; Ilya Bykov; Igor Tsymbalyuk; Mikhail Bykov;

The dynamic activities of glutathione reductase (GR) and glutathione peroxidase (GPO), as well as the concentration of reduced glutathione (GSH), were examined at both the organ and systemic levels in a liver ischemia-reperfusion model. Samples were collected at 5 min intervals from rats (n=95) during a 20 min ischemic period and after 20 min reperfusion. There was an adaptive increase (30–50 %) in GR activity and GSH concentration in erythrocytes during the ischemic period. Within 10 min of ischemia, there was a 1.5- to 2-fold decrease in all parameters measured in the liver tissue. Liver reperfusion was accompanied by greater decreases in GR and GPO activities, as well as increased GSH concentrations, in the erythrocytes. In liver tissue, against the background of increased oxygen toxicity, there was a 2- to 3-fold increase in GPO activity. GSH concentration and the GR activity remain decreased during 10 min reperfusion but then returned to control levels. These findings provide information about the pathogenesis of ischemic reperfusion and also illustrate pathobiochemical links that are potentially subject to beneficial metabolic correction.

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Keywords: ischemia-reperfusion syndrome, liver, antioxidant system, glutathione


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