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Characterization of the metabolic disorders in rats with alloxan-induced diabetes and chronic alcoholic intoxication

[Experimental medicine]
Ilya Bykov; Konstantin Popov; Hristina Berberidi; Igor Tsymbalyuk; Galina Ermakova; Ivan Pavlyuchenko; Petr Storozhuk; Viktor Ovsyannikov;

We investigated the metabolic disorders in rats with alloxan-induced diabetes and chronic alcoholic intoxication. Rats were divided into four groups: the first group was untreated controls, the second group was administered alloxan to induce diabetes, the third consumed ethanol for 2 months, and the fourth group consumed ethanol for 2 months and were injected with alloxan half way through this period. Plasma glucose concentrations in rats with both alcoholic intoxication and alloxaninduced diabetes were 22 % higher than in those with alloxan-induced diabetes alone. Plasma ALT activity was 1.7 and 2.2 times higher in rats in groups 3 and 4, respectively, than in control rats. Rats in group 4 showed a 30 % higher GR activity and 29 % and 20 % reductions in CAT and SOD activities in erythrocytes, respectively, versus controls. In addition, the concentration of GR in erythrocytes was a mean 31 % lower in rats in groups 2–4 than in controls. These data reveal more severe disruption of carbohydrate metabolism and a more severe imbalance in the antioxidant system in rats with diabetes mellitus if they have already been subjected to chronic alcohol intoxication.

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Keywords: alloxan diabetes, alcohol, oxidative stress, antioxidant system


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