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https://doi.org/10.14300/mnnc.2018.13062

Assessment of indicators of the thiol metabolism of blood plasma of patients with inflammatory diseases of the small pelvis organs at antioxidant correction

[Experimental medicine]
Ilya Bykov; Konstantin Popov; Inna Egorova; Aleksandr Storozhuk;

The study has demonstrated the possibility of increasing the informative value of the determination of the thiol group content in patients with inflammatory diseases of the small pelvis organs. The content of easily accessible and inaccessible thiol groups was determined in blood plasma with the aid of the Ellman reagent with the subsequent calculation of their ratio. The experiment has been performed with intact biological material or after exposure to hydrogen peroxide in the in vitro test system. The investigation of the content and ratio of different fractions of thiol groups showed an increase of the fraction of easily accessible SH-groups. In patients of different groups an increased amount of thiol groups oxidized by peroxide was recorded, which indirectly indicates a decreased antioxidant activity. Analysis of the therapy effectiveness in patients with chronic endometritis with bilateral salpingo-oophoritis showed the greatest efficacy of sodium thiosulfate, vitamins A and E in addition to standard therapy.

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Keywords: thiol groups, thiol-containing proteins, Ellman’s reagent, antioxidants