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https://doi.org/10.14300/mnnc.2018.13022

Influence of carnitine chloride on mitochondria of the heart of rats during the modeling of hyperhomocysteinemia

[Experimental medicine]
Valentina Zvyagina; Eduard Belskikh; Oleg Uryasev; Dmitriy Medvedev; Valentina Kiseleva; Ludmila Tverdova;

The aim of the study was to investigate the effects of carnitine chloride on the functional state of rat heart mitochondria on the background of experimental hyperhomocysteinemia in rats. Modeling of hyperhomocysteinemia was carried out by intragastric injection of methionine containing suspension at the dose of 3 g/kg per day with adding methionine to the drinking water for 21 days. The mitochondria of cardiomyocytes were isolated where the activity of lactate dehydrogenase and superoxide dismutase, the concentration of lactate, carnitine and carbonyl derivatives of proteins were measured. It was discovered that the modeling of hyperhomocysteinemia in rats creates prerequisites for the development of mitochondrial dysfunction in the form of a sharp decrease in carnitine concentration, accumulation of lactate and increasing in the mitochondria requirement for antioxidant protection due to oxidative stress development. The carnitine chloride on the background of modeling of hyperhomocysteinemia reduces the severity of hyperhomocysteinemia, and also helps to decrease the manifestations of mitochondrial myocardiocyte dysfunction.

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Keywords: homocysteine, mitochondrial dysfunction, carnitine, oxidative stress, cardiomyocytes