Medical news
of the North Caucasus
Scientific journal
Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137

Site search

Correspondence address
310 Mira Street, Stavropol, Russia, 355017

+7 8652 352524; +7 8652 353229.

+7 8652 352524.


Interrelation of endothelial nitric oxides synthase activity in tissues of the stomach and magnesium balance in the period of erosive-ulcerative acid-induced lesion development in rats with different resistance to stress

[Experimental medicine]
Lyudmila Rogova; Victoria Povetkina;

Acetate stomach ulcer was experimentally modeled in stress resistant and stress nonresistant rats for immunohistochemical identifying a specific number and expression intensity of eNOS-positive cells and determination of the magnesium level in biological media. The content of intra-erythrocyte magnesium in reaction with titanium yellow was reduced in rats with different resistance to stress in low eNOS activity in the gastric mucosa, in the muscular layer in stress nonresistant animals and almost complete absence of antigen-positive cells in the submucosa. A positive correlation was found between the level of intra-erythrocyte magnesium and the specific number of eNOS-positive cells in the gastric mucosa in both groups of animals and also between expression intensity in the submucosa of stress nonresistant rats. A relationship was established between the magnesium content, specific number and expression intensity of eNOS in stress nonresistant rats in the stomach submucosa.


1. Adamsson J., Ottsjö L. S., Lundin S. B., Svennerholm A.-M., Raghavan S. Gastric expression of IL-17A and IFNγ in Helicobacter pylori infected individuals is related to symptoms. Cytokine. 2017;99:30-34. doi: 10.1016/j.cyto.2017.06.013
2. Alderton W. K., Cooper C. E., Knowles R. G. Nitric oxide synthases: Structure, function and inhibition. Biochemical Journal. 2001;357(3):593-615. doi: 10.1042/0264-6021:3570593
3. Alexander R. T., Hoenderop J. G., Bindels R. J. Molecular determinants of magnesium homeostasis: Insights from human disease. Journal of the American Society of Nephrology. 2008;19(8):1451-8. doi: 10.1681/ASN.2008010098
4. Han T., Tang Y., Li J., Xue B., Gong L. [et al.] Nitric oxide donor protects against acetic acid-induced gastric ulcer in rats via S-nitrosylation of TRPV1 on vagus nerve. Scientific Reports. 2017;7(1):2063. doi:10.1038/s41598-017-02275-1
5. Kamyshnikov V. S. Spravochnik po klinikobiokhimicheskim issledovaniyam i laboratornoy diagnostike. Moscow: «MEDPress-Inform», 2004.
6. Korzhevsky D. E., Kirik O. V., Karpenko M. N., Petro-va E. S., Grigoryev I. P., [et al.] Teoreticheskiye osnovy i prakticheskoye primeneniye metodov immunogistokhimii: ruk-vo pod red. D. E. Korzhevskogo. Spec.Lit., 2014.
7. Lundberg J. O., Weitzberg E. Biology of nitrogen oxides in the gastrointestinal tract. Gut. 2013;62(4):616-629. doi: 10.1136/gutjnl-2011-301649
8. Magnesium in the Central Nervous System. Edited by R. Vink, M. Nechifor. Adelaide: University of Adelaide Press, 2011, 355. doi:10.1017/UPO9780987073051
9. Okabe S., Miyake H., Yamasaki S. Effects of NC-1300, a gastric proton pump inhibitor, on healing of acetic acid-induced gastric ulcers in rats. Digestive Diseases and Sciences. 1989;34(7):1035-1042. doi: 10.1007/BF01536370
10. Raghavan S., Holmgren J., Svennerholm A.-M. Helicobacter pylori Infection of the Gastric Mucosa. Mucosal Immunology. 2015;1-2:985-1001. doi:10.1016/B978-0-12-415847-4.00051-3
11. Rogova L. N., Shesternina N. V., Zabolotneva K. O., Staravoytov V. A. Agressivnye faktory i mekhanizmy zashchity tkaney zheludochno-kishechnogo trakta, ikh rol v patologii. Volgograd: Publ. house «VolgGMU», 2014.
12. Sung J. J. Y., Kuipers E. J., El-Serag H. B. Systematic review: The global incidence and prevalence of peptic ulcer disease. Alimentary Pharmacology and Therapeutics. 2009;29(9):938-946. doi: 10.1111/j.1365-2036.2009.03960.x
13. Zabala T. B., Lucero Y., Lagomarcino A. J., Orellana-Manzano A., George S. [et al.] Review: Prevalence and dynamics of Helicobacter pylori infection during childhood. Helicobacter. 2017;22(5):e12399. doi: 10.1111/hel.12399

Keywords: endothelial nitric oxide synthase, magnesium, acetate ulcer, stress resistant, stress nonresistant rats

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy