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ISSN 2073-8137

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Cathepsin as a possible way of adaptation of vascular wall to oxidative stress in conditions of ischemia and reperphy

[Experimental medicine]
Roman Kalinin; Alexandr Pshennikov; Yuliya Abalenikhina; Igor Suchkov; Nina Mzhavanadze; Sergey Isakov;

Vascular surgeons routinely deal with ischemia and reperfusion injury, a process similar to inflammation, i.e. leading to increased production of active forms of oxygen and inflammatory mediators.

The objective of this research is evaluation and comparison of oxidative stress in arterial wall in ischemiareperfusion injury.

In laboratory animals (rats of the Wistar line) a model of ischemia and reperfusion was developed, followed by the determination of the activity of lysosomal proteinases and the level of oxidation-modified proteins in the vascular wall homogenates and in an intact wall more distal to the surgical intervention, at the time 1, 3, 5, 7 days in both groups, respectively.

The obtained results demonstrate the development of oxidative stress in ischemia-reperfusion from 1 to 7 days, and in ischemia – on days 3 and 5. With ischemia and ischemia-reperfusion, the reserve-adaptive potential is depleted, however, primary markers predominate in ischemia, and the secondary – in reperfusion. A positive correlation was proved between the oxidative carbonylation of proteins and the activity of cathepsins B and L in the wall of the vessel.


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Keywords: ischemia, reperfusion, vascular wall, lysosomal proteases, oxidation-modified proteins

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy