Site search
Correspondence address
310 Mira Street, Stavropol, Russia, 355017
Tel
+7 865 2352511, +7 865 2353229.
E-mail
medvestnik@stgmu.ru
The journal is included into The list of leading scientific periodicals.
The journal is included into VINITI database and is registered in Electronic scientific library.
The journal is indexed by SCOPUS, Ulrich's International Periodicals Directory.
[Oncology]
Dmitriy Vodolazhsky; Sergey Dimitriadi; Denis Kutilin; Elena Frantsiyants; Oleg Kit;
Currently, to search markers of susceptibility to ischemia and postreperfusion injury of kidney during nephron sparing surgery in terms of warm renal ischemia, as well as to determine fundamental molecular biological processes that underlie this phenomenon, it is appropriate to study the expression of apoptosisregulating genes. By quantitative real-time PCR was performed determination of the relative expression of 14 genetic loci (MDM 2, BAX, CASP7, CASP3, CASP8, CASP9, BCL2, CASP8/FADD, p53, APAF1, AIFM1, ICAD, XIAP, HIF1A). The study identified genes – CASP3, CASP8, CASP9, CASP8/FADD and HIF1A, whose expression allows prediction of the acute renal injury development in patients with cancer after nephronsparing surgery in terms of warm renal ischemia.
References:
1. Kit O. I., Frantsiyants E. M., Dimitriadi S. N., Kaplieva I. V., Trepitaki L. K. Eksperimentalnaya urologiya. – Experimental Urology. 2014;(4):12-15.
2. Kogan M. I., Chibichyan M. B., Vodolazhsky D. I. Klinicheskaya onkologiya. – Clinical Oncology. 2012;(5):59-60.
3. Matveev V. B., Perlin D. V., Figurin K. M., Volkova M. I. Prakticheskaya onkologiya. – Practical Oncology. 2005;6(3):162-166.
4. Becker F. European Urology. 2009;56:625-635.
5. Burne M. J., Haq M., Matsuse H., Mohapatra S., Rabb H. Transplantation. 2000;69(5):1023-1025.
6. Chomczynski P., Sacchi N. Nat. Protoc. 2006;1(2):581-585.
7. Eckardt K.-U., Bernhardt W. W., Weidemann A., Warnecke Ch., Rosenberger Ch., Wiesener M. M., Willam C. Kidney International. 2005;68:46-51.
8. Lee J. W., Bae S. H., Jeong J. W., Kim S. H., Kim K. W. Experimental & Molecular Medicine. 2004;36(1):1-12.
9. Schmittgen T. D., Livak K. J. Nature Protocols. 2008; 3:1101-1108.
10. Sims N. R., Muyderman H. Biochimica et Biophysica Acta. 2010;1802(1):80-91.
11. Tompson R. H., Lane B. R., Lohse C. M., Leibovich B. C., Fergany A., Frank I., Gill I. S., Blute M. L., Campbell S. C. Urology. 2012;79(2):356-360.
12. Wiener C. M., Booth G., Semenza G. L. Biochemical and Biophysical Research Communications. 1996;225(2):485-488.
Keywords: gene expression, ischemic and postreperfusion renal damage, warm renal ischemia, prognosis