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ISSN 2073-8137
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Assesment of microbiological status in children with abnormalities of dental system by the results bakteriologis of molecular genetic research

[Original research] [Stomatology]
Dmitry Domenyuk; Vladimir Zelenskiy; Anna Karslieva; Igor Bazikov;

The method of bacteriologic investigation employing the anaerobic cultivation technique, as well as the molecular-genetic method based on polymerase chain reaction were used to detect the major parodontopathogenic species and to conduct quantitative evaluation of parodontopathogenic and residential microflora in gingival sulcus biofilm of children with dentoalveolar anomalies. It has been shown that a proper indicator best reflecting the intensity of morphological and functional disturbances, is an increase of parodontopathogenetic markers (Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Bacteroides forsythus) in gingival fluid.

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Keywords: molecular-genetic method, anaerobic cultivation, dentoalveolar anomalies, parodontopathogenic microflora


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