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https://doi.org/10.14300/mnnc.2024.19058

Еffect of monoclonal antibodies to neuroreceptors on cyclic AMP contents in rat lymphocytes

[Abstracts]
Mariya Baturina; Ekaterina Vladimirovna Grudina; Eduard Beyer;

The study implied analyzing the effect that monoclonal antibodies (IgG) to dopamine receptors Type 1 and 2 (DR1 and DR2) and NMDA receptors (NR2A NR2B subunits) have on the cyclic AMP (c-AMP) levels of isolated lymphocytes. Per each antibody there were solutions prepared at concentrations of 3 ng/ml, 30 ng/ml and 300 ng/ml, as well as the Antibody combinations were prepared: NR2A+DR1; NR2A+DR2; NR2B+DR1; NR2B+DR2. Solutions with the concentration of each antibody were prepared for each combination: 3x300 ng/ml; 30x30 ng/ml; 300x3 ng/ml. Isolated blood lymphocytes of healthy rats were treated in vitro with solutions of antibodies and their combinations. The control series of experiments relied on a solution containing no antibodies. All solutions containing individual antibodies had a greater reducing effect on the c-AMP level in lymphocytes if compared to the control series. The most significant decrease in c-AMP was obtained using antibodies to NR2A at concentrations of 30 and 300 ng/ml, antibodies to NR2B 3 and 300 ng/ml. Solutions of antibodies to DR1 reduced c-AMP more strongly than antibodies to DR2. A combination of antibodies in solutions resulted in a mutually enhanced or weakened effect. Potentiation of the effect of antibodies with a significant decrease in c-AMP levels was identified when using NR2A+DR2 at concentrations of 3x300 ng/ml. The effect of antibodies on the c-AMP level could be eliminated through using antibodies to NR2A+DR1 (concentrations: 30x30 ng/ml) and NR2B+DR1 (300x3 ng/ml). In these cases, the c-AMP levels in lymphocytes featured no difference from the control series of experiments. In case there was a combination of NR2A+DR2 antibodies at a concentration of 30x30 ng/ml, the effect on the c-AMP content proved weakened.

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References:
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5. Baturina M. V. Analysis of the effect of serum autoantibodies to neuroreceptors on changes in the concentration of cyclic AMP in isolated rat lymphocytes. Meditsinskii vestnik Severnogo Kavkaza. – Medical News of North Caucasus. 2024;19(1):62-64. (In Russ.). https://doi.org/10.14300/mnnc.2024.19015

Keywords: antibodies, NMDA receptors, dopamine receptors, lymphocytes, cyclic AMP