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https://doi.org/10.14300/mnnc.2023.18038

Dynamics of indicators of the prooxidant-antioxidant system in the blood and exudate under the treatment of purulent wounds with negative pressure and silver nanoparticles

[Experimental medicine]
Vadim Vladimirovich Malyshko; Alexander Basov; Anna Anatolievna Dorohova; Arkady Viktorovich Moiseev; Oleg Vyacheslavovich Dyakov; Ivan Pavlyuchenko; Aleksandr Storozhuk;

The study determined the effect of silver nanoparticles (AgNPs) in the treatment of purulent wounds with negative pressure instillations (NPWTi) on the parameters of the antioxidant system of blood and exudate. The study was conducted on 72 laboratory animals, which were divided into four equal groups. In contrast, in 3 groups, it was performed by modeling a purulent wound with its further treatment in various ways. In group 1, the traditional treatment method was used under a bandage with a levomekol; in group 2, wound treatment was carried out using a preparation with AgNPs; in group 3, the NPWTi method was used. In group 4 (control), modeling of a purulent wound was not performed. During the experiment, a study was made of the parameters of the enzyme link of antioxidant protection (catalase and superoxide dismutase), as well as the total antioxidant activity due to the content of low molecular weight substrates. It should be noted that only in animals of group 3, the indicator of antioxidant activity of the blood reached the values of the control group on the 12th day. In contrast, in group 2 it remained significantly reduced by 13.2 %. The data obtained indicate the ability of AgNPs to increase the prooxidant load in the wound area mainly in the first six days, with the development of an imbalance in the work of enzymes of the 1st and 2nd lines of antioxidant protection. This may indicate the advisability of the combined use of AgNPs and NPWTi in treating the first phase of the wound process.

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Keywords: wound process, silver nanoparticles, negative pressure instillations, wound treatment, oxidative stress, catalase, superoxide dismutase