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Colonocytes immunopositive to fatty acid synthase and CD3 in inflammatory bowel di

[Internal diseases]
Maria Mikhailovna Kudishina; Irina Kozlova; Anna Leonidovna Pakhomova; Yulia Sergeevna Krylova;

Inflammatory bowel diseases (IBD) – ulcerative colitis (UC) and Crohn’s disease (CD) – are an urgent problem of modern gastroenterology. In IBD, the integrity of the cell membrane of colonocytes, the main components of which are phospholipids, is violated. Changes in the optical density and relative area of expression of colonocytes immunopositive to fatty acid synthase (FAS) involved in the synthesis of structural units of the cytolemma can quantitatively reflect violations of the cell permeability of the intestinal barrier. The quantitative density of CD3-immunopositive colonocytes reflects the degree of immune damage to the gut in IBD. The aim of the study was to analyze the morphometric characteristics of colonocytes immunopositive to FAS and CD3 in UC and CD in comparison with the clinical features of the diseases. 94 patients were examined (32 with CD, 62 with UC). Significant changes in the relative area and optical density of the expression of colonocytes that are immunopositive to FAS were found to correlate with the severity of the IBD attack. The quantitative density of CD3-immunopositive colonocytes in the colon mucosa in IBD increases with continuously recurrent course, as well as in the presence of extra-intestinal manifestations in UC.

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Keywords: fatty acid synthase, CD3-immunopositive colonocytes, ulcerative colitis, Crohn’s disease


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