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Cerebroprotective properties of chrysin and chryzantemine under conditions of the focal cerebral ischemia

[Experimental medicine]
Andrei Voronkov; Dmitry Pozdnyakov; Siranush Arturovna Nigaryan;

The aim was to study the cerebroprotective properties of chrysin and chrysanthemum under conditions of focal cerebral ischemia. The study was conducted on 30 male Wistar rats weighing 250–300 g. Ischemic brain damage was reproduced by irreversible occlusion of the right middle cerebral artery. Mexidol (100 mg/kg, Moskhimpharmpreparaty, Russia) was chosen as a reference drug. The concentration of lactic and pyruvic acids was determined by an enzymatic colorimetric method using a standard set of reagents produced by NPF Arbis+ (St. Petersburg, Russia). To assess homocysteine content in the blood plasma a standard DiaSys reagent kit (Germany) was used. The degree of the brain hydration was determined by the method of drying. Zone of cerebral necrosis was assessed by triphenyltetrazolium method. The study showed that administration of chrysin and chrysanthemum decreases the activity of synthesis of lactate (7.94 % (p<0.05) and 12.83 % (p<0.05)), pyruvate (105.04 % (p<0.05) and 51.23 % (p<0.05)), homocysteine (13.96 % (p<0.05) and 26.75 % (p<0.05)), and also helps to reduce the severity of necrosis (86.6 % (p<0.05) and 39.45 % (p<0.05)) and cerebral edema (9.37 (p<0.05) and 11.68 % (p<0.05)), in comparison with the group of negative control. The studied compounds are superior to the reference drug in their effect on the pyruvate synthesis (37.47 % reduction by chrysin (p<0.05), 18.52 % – by chrysanthemum (p<0.05)) in comparison to the group of Mexidol. It should be noted that Mexidol (a decrease of 83.4 %; p<0.05) and chrysin (a decrease of 86.6 % (p<0.05) almost equally contribute to a decrease in the severity of brain tissue necrosis in comparison with the negative control group. Thus, a further in-depth study of the cerebroprotective properties of chrysin and chrysanthemum seems to be promising


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Keywords: ischemia, ischemic stroke, flavonoids, chrysin, chrysanthemine

Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy