logo
Medical news
of the North Caucasus
Scientific journal
Mass media registration certificate dated December 7, 2006.
Series ПИ #ФС 77-26521.
Federal service for surveillance over non-violation of the legislation in the sphere of mass communications and protection of cultural heritage.
ISSN 2073-8137
rus
русский
eng
english

Site search



Correspondence address
310 Mira Street, Stavropol, Russia, 355017

Tel
+7 865 2352511, +7 865 2353229.

E-mail
medvestnik@stgmu.ru

Study of cefotaxime influence on morphological changes in the cells of Staphylococcus aureus by atomic force microscopy

[Clinical Pharmacology]
Vladimir Baturin; M. Selimov; Albert Bolatchiev; Roman Budkevich; Vladimir Sadovoy; Elena Kunitsyna;

With the use of high-resolution atomic force microscopy the effect of cefotaxime on morphological and mechanical properties of gram-positive bacteria (Staphylococcus aureus) cells was studied. The difference in the morphological features of bacterial populations was shown by the nature of the response to the antimicrobial agent. The common point was a decrease in diameter and an increase in the altitude characteristics of cells, which may be due to internal osmotic pressure on the cell wall, which reduced its rigidity under the action of cefotaxime.

Download

References:
1. Baturin V. A., Shchetinin Ye. V., Demidenko I. F., Korableva O. A. Byulleten antibiotikorezistentnosti respiratornykh patogenov v OITAR g. Stavropolya. Stavropol: StGMU, 2014.
2. Erokhin P. S. Atomno-silovaya mikroskopiya kak instrument opredeleniya chuvstvitelnosti baktery k faktoram bioticheskoy i abioticheskoy prirody: avtoref. dis. kand. fiz.-mat. Nauk. Saratov, 2015.
3. Mironov V. L. Osnovy skaniruyushchey zondovoy mikroskopii. N. Novgorod, 2004.
4. Opredeleniye chuvstvitelnosti mikroorganizmov k antibakterialnym preparatam. Metodicheskiye ukazaniya. M., 2004.
5. Pleskova S. N. Dubrovin Ye. V., Golubeva I.S., Gorshkova Ye. N., Pudovkina Ye. E. Fizika tvyordogo tela. Vestnik Nizhegorodskogo universiteta im. N. I. Lobachevskogo. – Solid state physics bulletin of the Nizhny Novgorod University, N. I. Lobachevsky. 2013;2(2):34-38.
6. Prakticheskoye rukovodstvo po antiinfektsionnoy khimioterapiii. pod red. L. S. Strachunskogo, Yu. B. Belousova, S. N. Kozlova. Smolensk: «MAKMAKh», 2007.
7. Ratsionalnaya antimikrobnaya terapiya. pod red. S. V. Yakovleva. 2-e izd., pererab. i dop. M.:«Litterra», 2015.
8. Yaminsky I. V., Demin V. V., Bondarenko V. M. Zhurn. mikrobiologii. – Journal of microbiology. 1997;6:15-18.
9. Binnig G., Quate C. F., Gerber Ch. Phys Rev Lett. 1986;6(59):930-933.
10. Bondareva N. I., Timchenko L. D., Rzhepakovsky I. V. Res. J. Pharm., Biol. Chem. Sci. (RJPBCS). 2016;7(6):1224- 1230.
11. Finberg R. V., Moellering R. C., Tally F. P. Clin. Infect. Dis. 2004;(39):1314-1320.
12. Fuhrmann A., Ros R. Nanomedicine. 2010;5(4):657-665.
13. Fuchs P. C., Jones R. N., Thornsberry C., Barry A. L., Gerlach E. H., Sommers H. M. Antimicrob. Agents Chemother. 1981;20(6):747-759.
14. LeFrock J. L., Prince R. A., Leff R. D. Pharmacotherapy. 1982;2(4):174-184. doi: 10.1002/j.1875-9114.1982. tb03185
15. Martínez J. L., Baquero F. Ups. J. Med. Sci. 2014;119(2): 68-77. doi: 10.3109/03009734.2014.901444
16. Meyer R. L. Zhou X., Tang L. Ultramicroscopy. 2010;110(11):1349-1357. doi: 10.1016/j.ultramic. 2010.06.010
17. Nikiyan A., Vasilchenko A., Deryabin D. Intern. J. Microbiol. 2010. doi:10.1155/2010/704170
18. Volle C. B., Ferguson M. A., Aidala K. E., Spain E. M., Nunez M. E. Coll. Surf. B Biointerfaces. 2008;67(1): 32-40.
19. Saranya S., Hemashenpagam N. Sci. Res. 2011;2(4): 528-534.
20. Smirnova M. V., Lubenko I. Yu. Intern. J. Antimicrobial Agents. 2007;29(2):165-169.

Keywords: cefotaxime, atomic force microscopy, Staphylococcus aureus, bacterial cells morphometry


Founders:
Stavropol State Medical Academy
Pyatigorsk State Research Institute of Balneotherapeutics
Pyatigorsk State Pharmaceutical Academy