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https://doi.org/10.14300/mnnc.2015.10067

Estimation of the influence of medium with different isotopic D/H composition on DNA lymphocytes repair

[Experimental medicine]
Elena Tekutskaya; Stepan Dzhimak; Alexander Basov; Ekaterina Barisheva; Sergey Fedosov;

The influence of deuterium depleted water (40 ppm) on lymphocyte DNA repair system was investigated. The experiment was performed on lymphocytes isolated from blood of healthy people and patients with congenital malformations of the maxillofacial region, in the lysates of which there was determined the number of single-strand DNA breaks. Incubation of pure suspension of lymphocytes was performed in 0.9 % solution of NaCl, prepared on water with various concentrations of deuterium (40 and 150 ppm). The number of single-strand DNA breaks was evaluated by the ratio of the amount of fluorescence of control and experimental samples. According to the experiment it was found that incubation in solution with deuterium depleted water of lymphocytes obtained from the blood of the patients with congenital malformations of the maxillofacial area, reduces the number of single-strand DNA breaks by 21–24 %. It has been shown that incubation of lymphocytes in solutions with deuterium depleted water activates DNA repaire system.

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Keywords: deuterium depleted water, lymphocytes, single-strand DNA breaks, cell repair