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ISSN 2073-8137
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Effect of Bacillus anthracis strains culture conditions on their phagosensitivity

[Original research] [Infectious diseases]
Tatyana Golovinskaya; Olga Tsygankova;

Effect of individual characteristic of B. anthracis strains capsule formation is studied in various culture conditions on sensitivity to seven specific anthrax bacteriophages: Gamma А-26, Fah-ВНИИВВиМ, R/DPh-6, 186, ВА-9, Saratov, K-ВИЭВ was studied. It has been established that typical B. anthracis strains, forming R-type colonies in acapsular form were sensitive to all studied bacteriophages, unlike strains forming OSS-type colonies which were not lysed by bacteriophages Fah-ВНИИВВиМ, K-ВИЭВ, Saratov and 186 in such conditions which is also connected with some changes in the structure of surface structures of bacterial cells and with biological features of mentioned bacteriophages. All strains in the capsular form, irrespective of individual capsule formation requirements, were insensitive to the lytic action of specific anthrax bacteriophages. When carrying out indication and identification of B. anthracis with the use of preparations of specific anthrax bacteriophages it should be taken into consideration the possibility of existence of atypical strains capable of forming a capsule on usual cultural media in air atmosphere that makes them insensitive to the lytic action of bacteriophages.

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Keywords: culture conditions, Bacillus anthracis strains, capsule formation, phagosensitivity


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